Matches in SemOpenAlex for { <https://semopenalex.org/work/W2468765904> ?p ?o ?g. }
- W2468765904 endingPage "90" @default.
- W2468765904 startingPage "84" @default.
- W2468765904 abstract "Ionic liquids (ILs) have many potential benefits in biochemical processes, including improved substrate or product solubility, increased enzyme selectivity, and higher yield. Varying ion substituents allow ILs to be tuned or optimized to achieve a specific goal. Unfortunately, optimization based on a single design criterion can have undesirable side effects on other process components. For example, hydrophilic ILs capable of efficiently dissolving biomass often inhibit enzymatic activity during hydrolysis. A panel of nine different aqueous ILs was selected for this study to systematically assess which factors contribute to the loss of enzyme activity. The activity of endoglucanase E1 from Acidothermus cellulolyticus steadily decreased in higher concentrations of ILs, especially in the presence of the common cellulose dissolving solvent 1-ethyl-3-methylimidazolium acetate. The impact of most other ILs could be rationalized via the Hofmeister series. Enzyme behavior was further probed by rationally modifying the surface charge of E1. Variants were computationally designed to have positively or negatively charged surfaces and assessed for activity in ILs. Surprisingly, positive supercharging maintained wild type activity levels in ILs, while negative supercharging drastically reduced activity. Discrepancy between stability and activity measurements for some ILs indicated active site inhibition or other unique inactivation mechanisms might be crucial components to consider in future studies." @default.
- W2468765904 created "2016-07-22" @default.
- W2468765904 creator A5018035456 @default.
- W2468765904 creator A5044055229 @default.
- W2468765904 creator A5046192679 @default.
- W2468765904 creator A5068113528 @default.
- W2468765904 date "2016-10-01" @default.
- W2468765904 modified "2023-09-25" @default.
- W2468765904 title "Characterization of supercharged cellulase activity and stability in ionic liquids" @default.
- W2468765904 cites W1548178570 @default.
- W2468765904 cites W1605936672 @default.
- W2468765904 cites W1902389382 @default.
- W2468765904 cites W1970608006 @default.
- W2468765904 cites W1977502625 @default.
- W2468765904 cites W1997897747 @default.
- W2468765904 cites W1999773325 @default.
- W2468765904 cites W2000476654 @default.
- W2468765904 cites W2003139549 @default.
- W2468765904 cites W2003475384 @default.
- W2468765904 cites W2007258535 @default.
- W2468765904 cites W2011823820 @default.
- W2468765904 cites W2012119658 @default.
- W2468765904 cites W2023440735 @default.
- W2468765904 cites W2027026584 @default.
- W2468765904 cites W2034006833 @default.
- W2468765904 cites W2034552424 @default.
- W2468765904 cites W2038662535 @default.
- W2468765904 cites W2040669111 @default.
- W2468765904 cites W2041290199 @default.
- W2468765904 cites W2052592862 @default.
- W2468765904 cites W2063508779 @default.
- W2468765904 cites W2068527479 @default.
- W2468765904 cites W2073562518 @default.
- W2468765904 cites W2079386876 @default.
- W2468765904 cites W2080057469 @default.
- W2468765904 cites W2086800637 @default.
- W2468765904 cites W2090750768 @default.
- W2468765904 cites W2098696316 @default.
- W2468765904 cites W2100505319 @default.
- W2468765904 cites W2102136347 @default.
- W2468765904 cites W2106114574 @default.
- W2468765904 cites W2113803740 @default.
- W2468765904 cites W2120381575 @default.
- W2468765904 cites W2130503243 @default.
- W2468765904 cites W2139401346 @default.
- W2468765904 cites W2140018967 @default.
- W2468765904 cites W2140466011 @default.
- W2468765904 cites W2151721114 @default.
- W2468765904 cites W2165861896 @default.
- W2468765904 cites W2167056652 @default.
- W2468765904 cites W2170746519 @default.
- W2468765904 cites W2170753468 @default.
- W2468765904 cites W2171008023 @default.
- W2468765904 cites W2277578780 @default.
- W2468765904 cites W2318249996 @default.
- W2468765904 cites W2328301624 @default.
- W2468765904 cites W2335499313 @default.
- W2468765904 cites W3023319571 @default.
- W2468765904 cites W3141314898 @default.
- W2468765904 cites W4247783334 @default.
- W2468765904 cites W4293003482 @default.
- W2468765904 cites W4379350677 @default.
- W2468765904 cites W2018624236 @default.
- W2468765904 doi "https://doi.org/10.1016/j.molcatb.2016.05.008" @default.
- W2468765904 hasPublicationYear "2016" @default.
- W2468765904 type Work @default.
- W2468765904 sameAs 2468765904 @default.
- W2468765904 citedByCount "13" @default.
- W2468765904 countsByYear W24687659042017 @default.
- W2468765904 countsByYear W24687659042018 @default.
- W2468765904 countsByYear W24687659042019 @default.
- W2468765904 countsByYear W24687659042020 @default.
- W2468765904 countsByYear W24687659042021 @default.
- W2468765904 countsByYear W24687659042022 @default.
- W2468765904 countsByYear W24687659042023 @default.
- W2468765904 crossrefType "journal-article" @default.
- W2468765904 hasAuthorship W2468765904A5018035456 @default.
- W2468765904 hasAuthorship W2468765904A5044055229 @default.
- W2468765904 hasAuthorship W2468765904A5046192679 @default.
- W2468765904 hasAuthorship W2468765904A5068113528 @default.
- W2468765904 hasConcept C111368507 @default.
- W2468765904 hasConcept C127313418 @default.
- W2468765904 hasConcept C134121241 @default.
- W2468765904 hasConcept C141603559 @default.
- W2468765904 hasConcept C154881586 @default.
- W2468765904 hasConcept C155574463 @default.
- W2468765904 hasConcept C161790260 @default.
- W2468765904 hasConcept C178790620 @default.
- W2468765904 hasConcept C181199279 @default.
- W2468765904 hasConcept C184651966 @default.
- W2468765904 hasConcept C185592680 @default.
- W2468765904 hasConcept C191897082 @default.
- W2468765904 hasConcept C192562407 @default.
- W2468765904 hasConcept C21951064 @default.
- W2468765904 hasConcept C2777289219 @default.
- W2468765904 hasConcept C2779251873 @default.
- W2468765904 hasConcept C2780471494 @default.
- W2468765904 hasConcept C28212737 @default.