FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2469002912> ?p ?o ?g. }

• W2469002912 endingPage "1367" @default.
• W2469002912 startingPage "1353" @default.
• W2469002912 abstract "Canonical inflammasome activation induces a caspase-1/gasdermin D (Gsdmd)-dependent lytic cell death called pyroptosis that promotes antimicrobial host defense but may contribute to sepsis. The nature of the caspase-1-dependent change in plasma membrane (PM) permeability during pyroptotic progression remains incompletely defined. We assayed propidium(2+) (Pro(2+)) influx kinetics during NLRP3 or Pyrin inflammasome activation in murine bone marrow-derived macrophages (BMDMs) as an indicator of this PM permeabilization. BMDMs were characterized by rapid Pro(2+) influx after initiation of NLRP3 or Pyrin inflammasomes by nigericin (NG) or Clostridium difficile toxin B (TcdB), respectively. No Pro(2+) uptake in response to NG or TcdB was observed in Casp1(-/-) or Asc(-/-) BMDMs. The cytoprotectant glycine profoundly suppressed NG and TcdB-induced lysis but not Pro(2+) influx. The absence of Gsdmd expression resulted in suppression of NG-stimulated Pro(2+) influx and pyroptotic lysis. Extracellular La(3+) and Gd(3+) rapidly and reversibly blocked the induced Pro(2+) influx and markedly delayed pyroptotic lysis without limiting upstream inflammasome assembly and caspase-1 activation. Thus, caspase-1-driven pyroptosis requires induction of initial prelytic pores in the PM that are dependent on Gsdmd expression. These PM pores also facilitated the efflux of cytosolic ATP and influx of extracellular Ca(2+) Although lanthanides and Gsdmd deletion both suppressed PM pore activity and pyroptotic lysis, robust IL-1β release was observed in lanthanide-treated BMDMs but not in Gsdmd-deficient cells. This suggests roles for Gsdmd in both passive IL-1β release secondary to pyroptotic lysis and in nonlytic/nonclassical IL-1β export." @default.
• W2469002912 created "2016-07-22" @default.
• W2469002912 creator A5005464828 @default.
• W2469002912 creator A5022624579 @default.
• W2469002912 creator A5030377750 @default.
• W2469002912 creator A5038155532 @default.
• W2469002912 creator A5056861397 @default.
• W2469002912 creator A5090265173 @default.
• W2469002912 date "2016-07-06" @default.
• W2469002912 modified "2023-10-14" @default.
• W2469002912 title "Active Caspase-1 Induces Plasma Membrane Pores That Precede Pyroptotic Lysis and Are Blocked by Lanthanides" @default.
• W2469002912 cites W1580699825 @default.
• W2469002912 cites W1604448976 @default.
• W2469002912 cites W1811981061 @default.
• W2469002912 cites W1813258042 @default.
• W2469002912 cites W1832818975 @default.
• W2469002912 cites W1859128422 @default.
• W2469002912 cites W1864325817 @default.
• W2469002912 cites W1882905539 @default.
• W2469002912 cites W1971084572 @default.
• W2469002912 cites W1973260880 @default.
• W2469002912 cites W1974885063 @default.
• W2469002912 cites W1976976361 @default.
• W2469002912 cites W1978274182 @default.
• W2469002912 cites W1981740875 @default.
• W2469002912 cites W1984520240 @default.
• W2469002912 cites W1985538633 @default.
• W2469002912 cites W1987329700 @default.
• W2469002912 cites W1990577987 @default.
• W2469002912 cites W1994473516 @default.
• W2469002912 cites W2003605139 @default.
• W2469002912 cites W2008261126 @default.
• W2469002912 cites W2016855276 @default.
• W2469002912 cites W2021259961 @default.
• W2469002912 cites W2022882293 @default.
• W2469002912 cites W2025024815 @default.
• W2469002912 cites W2026950841 @default.
• W2469002912 cites W2029668693 @default.
• W2469002912 cites W2029913563 @default.
• W2469002912 cites W2031211461 @default.
• W2469002912 cites W2032370476 @default.
• W2469002912 cites W2034550137 @default.
• W2469002912 cites W2043661964 @default.
• W2469002912 cites W2044571296 @default.
• W2469002912 cites W2048136172 @default.
• W2469002912 cites W2050688486 @default.
• W2469002912 cites W2050819949 @default.
• W2469002912 cites W2054966786 @default.
• W2469002912 cites W2055727949 @default.
• W2469002912 cites W2056303166 @default.
• W2469002912 cites W2065777365 @default.
• W2469002912 cites W2067960353 @default.
• W2469002912 cites W2068619092 @default.
• W2469002912 cites W2072884571 @default.
• W2469002912 cites W2073999035 @default.
• W2469002912 cites W2077317536 @default.
• W2469002912 cites W2078443771 @default.
• W2469002912 cites W2079882002 @default.
• W2469002912 cites W2080827095 @default.
• W2469002912 cites W2082400315 @default.
• W2469002912 cites W2083859580 @default.
• W2469002912 cites W2085689390 @default.
• W2469002912 cites W2087232320 @default.
• W2469002912 cites W2091363184 @default.
• W2469002912 cites W2097714951 @default.
• W2469002912 cites W2098053666 @default.
• W2469002912 cites W2101235551 @default.
• W2469002912 cites W2102459023 @default.
• W2469002912 cites W2108195113 @default.
• W2469002912 cites W2118800225 @default.
• W2469002912 cites W2120531380 @default.
• W2469002912 cites W2127237840 @default.
• W2469002912 cites W2128999191 @default.
• W2469002912 cites W2130153302 @default.
• W2469002912 cites W2140996651 @default.
• W2469002912 cites W2141260882 @default.
• W2469002912 cites W2141621785 @default.
• W2469002912 cites W2143102054 @default.
• W2469002912 cites W2148761780 @default.
• W2469002912 cites W2149943918 @default.
• W2469002912 cites W2153180469 @default.
• W2469002912 cites W2159590959 @default.
• W2469002912 cites W2163449047 @default.
• W2469002912 cites W2166682453 @default.
• W2469002912 cites W2175794645 @default.
• W2469002912 cites W2300256671 @default.
• W2469002912 cites W2317391245 @default.
• W2469002912 cites W2323335926 @default.
• W2469002912 cites W2392032823 @default.
• W2469002912 cites W2418806051 @default.
• W2469002912 cites W4245966407 @default.
• W2469002912 doi "https://doi.org/10.4049/jimmunol.1600699" @default.
• W2469002912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4976007" @default.
• W2469002912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27385778" @default.
• W2469002912 hasPublicationYear "2016" @default.
• W2469002912 type Work @default.
• W2469002912 sameAs 2469002912 @default.
• W2469002912 citedByCount "131" @default.

• next