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• W2469164911 endingPage "6390" @default.
• W2469164911 startingPage "6377" @default.
• W2469164911 abstract "N(2)-methylguanosine is one of the most universal modified nucleosides required for proper function in transfer RNA (tRNA) molecules. In archaeal tRNA species, a specific S-adenosyl-L-methionine (SAM)-dependent tRNA methyltransferase (MTase), aTrm11, catalyzes formation of N(2)-methylguanosine and N(2),N(2)-dimethylguanosine at position 10. Here, we report the first X-ray crystal structures of aTrm11 from Thermococcus kodakarensis (Tko), of the apo-form, and of its complex with SAM. The structures show that TkoTrm11 consists of three domains: an N-terminal ferredoxinlike domain (NFLD), THUMP domain and Rossmann-fold MTase (RFM) domain. A linker region connects the THUMP-NFLD and RFM domains. One SAM molecule is bound in the pocket of the RFM domain, suggesting that TkoTrm11 uses a catalytic mechanism similar to that of other tRNA MTases containing an RFM domain. Furthermore, the conformation of NFLD and THUMP domains in TkoTrm11 resembles that of other tRNA-modifying enzymes specifically recognizing the tRNA acceptor stem. Our docking model of TkoTrm11-SAM in complex with tRNA, combined with biochemical analyses and pre-existing evidence, provides insights into the substrate tRNA recognition mechanism: The THUMP domain recognizes a 3'-ACCA end, and the linker region and RFM domain recognize the T-stem, acceptor stem and V-loop of tRNA, thereby causing TkoTrm11 to specifically identify its methylation site." @default.
• W2469164911 created "2016-07-22" @default.
• W2469164911 creator A5022023364 @default.
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• W2469164911 date "2016-06-20" @default.
• W2469164911 modified "2023-10-16" @default.
• W2469164911 title "Structural and functional analyses of the archaeal tRNA m²G/m²₂G10 methyltransferase aTrm11 provide mechanistic insights into site specificity of a tRNA methyltransferase that contains common RNA-binding modules" @default.
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• W2469164911 doi "https://doi.org/10.1093/nar/gkw561" @default.
• W2469164911 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5291279" @default.
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• W2469164911 hasPublicationYear "2016" @default.
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