Matches in SemOpenAlex for { <https://semopenalex.org/work/W2469373567> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W2469373567 abstract "NPPB3-(4-5-nitro-2-(3-phenylpropylamino) benzoic acid, as one of the most commonly used chloride channel blockers, is very essential for chloride channel studies. However, the role of NPPB in mediating apoptosis is uncertain. The purpose of this study was to investigate the apoptosis mechanism of NPPB in human cancer DU145 cell lines. Cell viability was examined by MTT assay. JC-1 fluorescent probe was used for detecting l membrane potential. Cell apoptosis was determined by flow method (FCM). The expression of apoptosis-related protein Caspase-3, Cleaved caspase-3, PARP, Cleaved PARP, Bcl-2, Mcl-1, Bcl-xl, Bcl-w and Bax was detected by western blotting. Cell proliferation rate was remarkably inhibited 2 h after treating with NPPB(50,100 μmol/L) (p<0.01); Flow cytometry method analysis showed that the apoptosis rate in each treated group were significantly higher, especially for the promotion of early apoptosis rate effect was significantly (P<0.05); Images of JC-1 showed NPPB led to the decrease of intracellular mitochondrial membrane potential; Western blot results showed that caspase-3/PARP signaling pathway was activated, while the expression of cleaved caspase-3, cleaved PARP was significantly increased (P<0.01).PARP, as substrate of caspase-3, was significantly decreased (P<0.05).Bcl-2, Bcl-xl compared with the control group had no significant difference, and anti-apoptotic protein Mcl-1 (P<0.05) and Bcl-w (P<0.01) decreased significantly, pro-apoptotic protein Bax (P<0.01) were obviously upregulated. Our findings indicated that NPPB could increase apoptosis in DU145 cell lines via mitochondria-related apoptotic pathway, and following results presented that ClC-3 Chloride Channels involved in the process of NPPB induced DU145 cells apoptosis." @default.
- W2469373567 created "2016-07-22" @default.
- W2469373567 creator A5004894610 @default.
- W2469373567 creator A5071206989 @default.
- W2469373567 date "2016-01-01" @default.
- W2469373567 modified "2023-10-02" @default.
- W2469373567 title "ClC-3 is Involved in NPPB-Induced Apoptosis in DU145 Prostate Cancer Cells" @default.
- W2469373567 cites W1964336258 @default.
- W2469373567 cites W1966928113 @default.
- W2469373567 cites W2001111112 @default.
- W2469373567 cites W2005980194 @default.
- W2469373567 cites W2022152850 @default.
- W2469373567 cites W2025312106 @default.
- W2469373567 cites W2164930481 @default.
- W2469373567 cites W2359834310 @default.
- W2469373567 cites W2392060979 @default.
- W2469373567 doi "https://doi.org/10.4172/2155-9929.s2-018" @default.
- W2469373567 hasPublicationYear "2016" @default.
- W2469373567 type Work @default.
- W2469373567 sameAs 2469373567 @default.
- W2469373567 citedByCount "2" @default.
- W2469373567 countsByYear W24693735672016 @default.
- W2469373567 crossrefType "journal-article" @default.
- W2469373567 hasAuthorship W2469373567A5004894610 @default.
- W2469373567 hasAuthorship W2469373567A5071206989 @default.
- W2469373567 hasBestOaLocation W24693735671 @default.
- W2469373567 hasConcept C121608353 @default.
- W2469373567 hasConcept C126322002 @default.
- W2469373567 hasConcept C190283241 @default.
- W2469373567 hasConcept C2776438761 @default.
- W2469373567 hasConcept C2779723316 @default.
- W2469373567 hasConcept C2780192828 @default.
- W2469373567 hasConcept C502942594 @default.
- W2469373567 hasConcept C54355233 @default.
- W2469373567 hasConcept C71924100 @default.
- W2469373567 hasConcept C86803240 @default.
- W2469373567 hasConceptScore W2469373567C121608353 @default.
- W2469373567 hasConceptScore W2469373567C126322002 @default.
- W2469373567 hasConceptScore W2469373567C190283241 @default.
- W2469373567 hasConceptScore W2469373567C2776438761 @default.
- W2469373567 hasConceptScore W2469373567C2779723316 @default.
- W2469373567 hasConceptScore W2469373567C2780192828 @default.
- W2469373567 hasConceptScore W2469373567C502942594 @default.
- W2469373567 hasConceptScore W2469373567C54355233 @default.
- W2469373567 hasConceptScore W2469373567C71924100 @default.
- W2469373567 hasConceptScore W2469373567C86803240 @default.
- W2469373567 hasLocation W24693735671 @default.
- W2469373567 hasOpenAccess W2469373567 @default.
- W2469373567 hasPrimaryLocation W24693735671 @default.
- W2469373567 hasRelatedWork W1588138630 @default.
- W2469373567 hasRelatedWork W2052103316 @default.
- W2469373567 hasRelatedWork W2062196172 @default.
- W2469373567 hasRelatedWork W2118642421 @default.
- W2469373567 hasRelatedWork W2593394863 @default.
- W2469373567 hasRelatedWork W2728077352 @default.
- W2469373567 hasRelatedWork W2780918947 @default.
- W2469373567 hasRelatedWork W3024235066 @default.
- W2469373567 hasRelatedWork W4292237497 @default.
- W2469373567 hasRelatedWork W4361813216 @default.
- W2469373567 hasVolume "s2" @default.
- W2469373567 isParatext "false" @default.
- W2469373567 isRetracted "false" @default.
- W2469373567 magId "2469373567" @default.
- W2469373567 workType "article" @default.