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• W2469696673 abstract "Food infections are among the most serious public health concerns and are one of the major causes of morbidity and mortality. Monitoring and separation of such contaminants is an instrumental component in understanding and managing risks to human health and the environment. Many researchers and engineers have indulged in this important and difficult task and have developed technologies aiming the detection and removal of pathogenic organisms in processed as well as raw food products. Application of nanotechnology for monitoring and separation of food-borne pathogens is an active area of research. The magnetic nanoparticles are introduced into conventional pathogen detection techniques to make them simple, rapid, highly selective, and sensitive. The principle employed is that magnetic nanoparticles are often immobilized with various biomolecules like antibodies that have high selectivity to target analytes. Due to their large specific surface area and specific bonding, the modified magnetic nanoparticles recognize and capture the analytes from crude samples to form a complex, which can be detected and separated quickly and efficiently. Biofunctional magnetic nanoparticles (BMNPs) are used to facilitate the rapid separation of Escherichia coli from beef, ground water, and milk samples. Magnetic nanoparticles when integrated with polymerase chain reaction (PCR), immunoassay, spectrometry, and biosensors, make a rapid or an online analysis/detection of pathogens. Superparamagnetic nanoparticles has improved the detection sensitivity of pathogens using PCR technique by 10–100 times. In this chapter, exploitation of magnetic nanoparticles for the monitoring and separation of various pathogens in the processed food and raw food materials including milk, meat, fruits, and vegetables has been detailed. The state of the art of sensor-based monitoring of microorganisms, strategies adopted to enhance the sensitivity of such devices, are also discussed. Recent advancements in the functionalization of magnetic nanoparticles for the specific detection and separation of various pathogens are also taken into account." @default.
• W2469696673 created "2016-07-22" @default.
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• W2469696673 date "2016-01-01" @default.
• W2469696673 modified "2023-10-14" @default.
• W2469696673 title "Monitoring and separation of food-borne pathogens using magnetic nanoparticles" @default.
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• W2469696673 doi "https://doi.org/10.1016/b978-0-12-804308-0.00009-1" @default.
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