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- W2469707054 abstract "Wound healing is a complex process where macrophages are highly involved, bothby clearing the tissue from bacteria and debris, but also by regulating the inflammationand healing outcome by production of various cell mediators. A characteristicfeature of macrophages is their ability to display a spectrum of different phenotypes.They can roughly be divided into two extreme phenotypes; M1 macrophages whichproduce pro-inflammatory cytokines and metalloproteinases and is strongly antimicrobialand M2 macrophages which produce anti-inflammatory cytokines, resolvinginflammation and promoting tissue repair. Although both phenotypes are key inwound healing, an imbalance of M1 macrophage domination can result in nonhealingulcers. At Molnlycke Health Care effort is being paid at developing wound careproducts that could enhance the healing. In order to develop such concepts, an invitro inflammatory model with M1/M2 macrophages could be utilized.The aim of the thesis is to identify and study the relation of cell mediatorsand markers related to M1/M2 polarization in cultured human monocyte-derivedmacrophages and also to create a theoretical link of relevance of chosen markers todata in murine models and the human diabetic ulcer.Monocytes were isolated from buffy coats, obtained from healthy blood donors.To induce M1 and M2 macrophages, cells were stimulated with LPS and IFN-(M1) or IL-4 (M2) for 6, 24, 48 or 72 hours prior to analysis. M1 cells where furtherreversibly polarized with IL-4 after 48 hours in order to switch phenotype from M1to M2 cells. Secreted markers of M1/M2 macrophages were studied with ELISA.Production of TNF- and IL-1 indicated a M1 phenotype and CCL18 productionindicated a M2 phenotype. Production of IL-10 which is a M2 phenotype marker wasproduced by M1-stimulated macrophages. No production of IL-23 and IL-1ra wasseen. Further, no significant CCL18 production was seen after reversible polarization,indicating that M1 macrophages did not switch phenotype to M2 macrophages.Different assay setups of lactate and reactive oxygen species production (M1 markers)did not display any significant results in this study." @default.
- W2469707054 created "2016-07-22" @default.
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- W2469707054 date "2016-01-01" @default.
- W2469707054 modified "2023-09-23" @default.
- W2469707054 title "In vitro culture of human monocytederived macrophages with regards to M1/M2 polarization" @default.
- W2469707054 hasPublicationYear "2016" @default.
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