FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2470036731> ?p ?o ?g. }

• W2470036731 endingPage "1063" @default.
• W2470036731 startingPage "1053" @default.
• W2470036731 abstract "Cordyceps has long been used to treat cancer. However, its pharmacologically active components as well as the molecular mechanisms underlying its effects are still unclear.To investigate the effect of MHP-1, a newly isolated polysaccharide from Mortierella hepialid (the asexual structure of C. sinensis), on breast cancer metastasis.The effect of MHP-1 on breast cancer cell migration, epithelial-mesenchymal transition (EMT) and TGF-β signaling were investigated in vitro and in vivo. The effect of MHP-1 against topotecan-resistant MCF-7 cells that developed an EMT-like phenotype was also examined.The in vitro effect of MHP-1 on breast cancer cell proliferation and migration was evaluated by CCK8 and transwell assay. Morphological changes were observed and EMT markers were detected by western blot. The production of MMPs was measured by quantitative PCR and ELISA assay. To further investigate the mechanism that MHP-1 inhibited breast cancer EMT, western blot, ELISA, luciferase reporter gene assay, siRNA, quantitative PCR, immunohistochemistry, and xenograft tumor model were performed.MHP-1 inhibited breast cancer cell migration but did not cause any cytotoxicity. MHP-1 significantly surpressed breast cancer EMT, and slightly decreased MMP-9 secretion. TGF-β signaling was selectively inhibited after MHP-1 treatment, and other EMT-related pathways, like Wnt and Notch, were not affected. MHP-1 reduced the secretion of TGF-β1, but rarely affected other EMT-induced cytokines. Dual luciferase assay and Smad2/3 phosphorylation analysis indicated that MHP-1 suppressed TGF-β signaling. We further showed that MHP-1 restored sensitivity in topotecan (TPT)-resistant MCF-7 cells that developed an EMT-like phenotype. Similarly, the effect of TPT on resistant MCF-7 cells was also increased either by ALK5 (TGFβRI) siRNA or by a small molecular inhibitor of ALK5, SB-431542. MHP-1 inhibited breast cancer metastasis in the MDA-MB-231 xenograft model, and the immunohistochemical staining showed dramatically decreased expression of ALK5 and vimentin, and increased expression of E-cadherin.MHP-1 significantly inhibited breast cancer metastasis and restored drug sensitivity in TPT-resistant cells via down-regulation of TGF-β signaling and EMT program. The combination of non-toxic agents like MHP-1 and current anti-cancer drugs should be considered in the future treatment of cancer." @default.
• W2470036731 created "2016-07-22" @default.
• W2470036731 creator A5003128598 @default.
• W2470036731 creator A5012392058 @default.
• W2470036731 creator A5018863416 @default.
• W2470036731 creator A5029619274 @default.
• W2470036731 creator A5033492731 @default.
• W2470036731 creator A5036293455 @default.
• W2470036731 creator A5058555021 @default.
• W2470036731 creator A5058775119 @default.
• W2470036731 creator A5063184651 @default.
• W2470036731 creator A5066135690 @default.
• W2470036731 creator A5083095344 @default.
• W2470036731 date "2016-09-01" @default.
• W2470036731 modified "2023-10-16" @default.
• W2470036731 title "MHP-1 inhibits cancer metastasis and restores topotecan sensitivity via regulating epithelial-mesenchymal transition and TGF-β signaling in human breast cancer cells" @default.
• W2470036731 cites W1832164599 @default.
• W2470036731 cites W1967734223 @default.
• W2470036731 cites W1973515006 @default.
• W2470036731 cites W1975389308 @default.
• W2470036731 cites W1975959672 @default.
• W2470036731 cites W1978615651 @default.
• W2470036731 cites W1979342940 @default.
• W2470036731 cites W1997786059 @default.
• W2470036731 cites W2000090486 @default.
• W2470036731 cites W2028593825 @default.
• W2470036731 cites W2042112143 @default.
• W2470036731 cites W2057757642 @default.
• W2470036731 cites W2062586437 @default.
• W2470036731 cites W2064312317 @default.
• W2470036731 cites W2064717188 @default.
• W2470036731 cites W2064964116 @default.
• W2470036731 cites W2069642304 @default.
• W2470036731 cites W2071749269 @default.
• W2470036731 cites W2072642940 @default.
• W2470036731 cites W2091892620 @default.
• W2470036731 cites W2100751052 @default.
• W2470036731 cites W2114293452 @default.
• W2470036731 cites W2130870648 @default.
• W2470036731 cites W2140551998 @default.
• W2470036731 cites W2141811703 @default.
• W2470036731 cites W2154589460 @default.
• W2470036731 cites W2157096396 @default.
• W2470036731 cites W2166423460 @default.
• W2470036731 cites W2297488593 @default.
• W2470036731 doi "https://doi.org/10.1016/j.phymed.2016.06.013" @default.
• W2470036731 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27444351" @default.
• W2470036731 hasPublicationYear "2016" @default.
• W2470036731 type Work @default.
• W2470036731 sameAs 2470036731 @default.
• W2470036731 citedByCount "21" @default.
• W2470036731 countsByYear W24700367312017 @default.
• W2470036731 countsByYear W24700367312018 @default.
• W2470036731 countsByYear W24700367312019 @default.
• W2470036731 countsByYear W24700367312020 @default.
• W2470036731 countsByYear W24700367312021 @default.
• W2470036731 countsByYear W24700367312022 @default.
• W2470036731 countsByYear W24700367312023 @default.
• W2470036731 crossrefType "journal-article" @default.
• W2470036731 hasAuthorship W2470036731A5003128598 @default.
• W2470036731 hasAuthorship W2470036731A5012392058 @default.
• W2470036731 hasAuthorship W2470036731A5018863416 @default.
• W2470036731 hasAuthorship W2470036731A5029619274 @default.
• W2470036731 hasAuthorship W2470036731A5033492731 @default.
• W2470036731 hasAuthorship W2470036731A5036293455 @default.
• W2470036731 hasAuthorship W2470036731A5058555021 @default.
• W2470036731 hasAuthorship W2470036731A5058775119 @default.
• W2470036731 hasAuthorship W2470036731A5063184651 @default.
• W2470036731 hasAuthorship W2470036731A5066135690 @default.
• W2470036731 hasAuthorship W2470036731A5083095344 @default.
• W2470036731 hasConcept C121608353 @default.
• W2470036731 hasConcept C126322002 @default.
• W2470036731 hasConcept C137620995 @default.
• W2470036731 hasConcept C137738243 @default.
• W2470036731 hasConcept C1491633281 @default.
• W2470036731 hasConcept C153911025 @default.
• W2470036731 hasConcept C185592680 @default.
• W2470036731 hasConcept C2779013556 @default.
• W2470036731 hasConcept C502942594 @default.
• W2470036731 hasConcept C530470458 @default.
• W2470036731 hasConcept C55493867 @default.
• W2470036731 hasConcept C62478195 @default.
• W2470036731 hasConcept C71924100 @default.
• W2470036731 hasConcept C76419328 @default.
• W2470036731 hasConcept C86803240 @default.
• W2470036731 hasConceptScore W2470036731C121608353 @default.
• W2470036731 hasConceptScore W2470036731C126322002 @default.
• W2470036731 hasConceptScore W2470036731C137620995 @default.
• W2470036731 hasConceptScore W2470036731C137738243 @default.
• W2470036731 hasConceptScore W2470036731C1491633281 @default.
• W2470036731 hasConceptScore W2470036731C153911025 @default.
• W2470036731 hasConceptScore W2470036731C185592680 @default.
• W2470036731 hasConceptScore W2470036731C2779013556 @default.
• W2470036731 hasConceptScore W2470036731C502942594 @default.
• W2470036731 hasConceptScore W2470036731C530470458 @default.
• W2470036731 hasConceptScore W2470036731C55493867 @default.
• W2470036731 hasConceptScore W2470036731C62478195 @default.
• W2470036731 hasConceptScore W2470036731C71924100 @default.

• next