FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2470461611> ?p ?o ?g. }

• W2470461611 abstract "Scytovirin is a novel anti-viral protein isolated from the cyanobacterium Scytonema varium. This protein has produced great scientific interest for its anti-human immunodeficiency virus (HIV) activity. Scytovirin disrupts the mechanism of HV infection by binding to HIV-1 proteins glycoprotein 120, glycoprotein 160, and glycoprotein 41. It does not bind to the CD4 receptor on human immune cells. The wild-type protein consists of a single 95 amino acid chain. When folded, Scytovirin’s amino acid sequence is arranged into two distinct domains, each with a high degree of sequence conservation. This implicates the importance of both domains in anti-HIV activity. Primers were designed and constructed to synthesize three novel, mutant, forms of the Scytovirin protein in an attempt to increase the anti-HIV activity of Scytovirin. Each gene analog featured coding to increase the size of the new mutant proteins by the addition of the second domain onto the nearly wild-type protein. The amino acid sequence connecting this domain was unique to each mutant. Single amino acids were replaced at numerous locations in each domain through site-directed mutagenesis to facilitate proper folding of the proteins. Once the gene constructs were sequenced and purified, all three mutants were expressed in Escheria coli, each with thrombin (THR) fused to its N-terminus. The protein constructs, three versions of SD1-SD2-SD2, were cleaved from THR with restriction enterokinase (rEK). Soluble protein was purified using polyhistidine tag affinity purification through FPLC, and HPLC. NMR determined the structures of the proteins and if proper folding had occurred. SD1-SD2-SD2 wild-type and short linkers were expressed in Origami cells, purified, and examined by 2D Heteronuclear NOESY NMR. NMR of SD1-SD2-SD2 wild-type linker indicated that the protein had not folded completely. Additional research involving the expression and purification parameters of SD1-SD2-SD2 is required to obtain proper folding before SD1-SD2-SD2 will be screened against HIV-1 to assess anti-viral activity." @default.
• W2470461611 created "2016-07-22" @default.
• W2470461611 creator A5082067868 @default.
• W2470461611 date "2013-01-01" @default.
• W2470461611 modified "2023-09-26" @default.
• W2470461611 title "Synthesis of a New Scytovirin Protein Derivative, Sd1-Sd2-Sd2, to Decrease Hiv-1 Binding Affinity" @default.
• W2470461611 cites W1546157935 @default.
• W2470461611 cites W1967703272 @default.
• W2470461611 cites W1977282539 @default.
• W2470461611 cites W1978298487 @default.
• W2470461611 cites W1981733052 @default.
• W2470461611 cites W1988930633 @default.
• W2470461611 cites W1995355944 @default.
• W2470461611 cites W2003749662 @default.
• W2470461611 cites W2005963291 @default.
• W2470461611 cites W2006771360 @default.
• W2470461611 cites W2007554184 @default.
• W2470461611 cites W2008972159 @default.
• W2470461611 cites W2014733693 @default.
• W2470461611 cites W2022058405 @default.
• W2470461611 cites W2023853526 @default.
• W2470461611 cites W2027743992 @default.
• W2470461611 cites W2029684104 @default.
• W2470461611 cites W2033757712 @default.
• W2470461611 cites W2041242149 @default.
• W2470461611 cites W2041386770 @default.
• W2470461611 cites W2057318373 @default.
• W2470461611 cites W2076877843 @default.
• W2470461611 cites W2086862822 @default.
• W2470461611 cites W2088458538 @default.
• W2470461611 cites W2088848851 @default.
• W2470461611 cites W2090381977 @default.
• W2470461611 cites W2099751883 @default.
• W2470461611 cites W2102237910 @default.
• W2470461611 cites W2109937808 @default.
• W2470461611 cites W2121527865 @default.
• W2470461611 cites W2139114486 @default.
• W2470461611 cites W2140665904 @default.
• W2470461611 cites W2148521301 @default.
• W2470461611 cites W2148927475 @default.
• W2470461611 cites W2156366212 @default.
• W2470461611 cites W2158322796 @default.
• W2470461611 cites W2159388972 @default.
• W2470461611 cites W2167377487 @default.
• W2470461611 cites W2170667639 @default.
• W2470461611 cites W287827804 @default.
• W2470461611 cites W340334483 @default.
• W2470461611 hasPublicationYear "2013" @default.
• W2470461611 type Work @default.
• W2470461611 sameAs 2470461611 @default.
• W2470461611 citedByCount "0" @default.
• W2470461611 crossrefType "dissertation" @default.
• W2470461611 hasAuthorship W2470461611A5082067868 @default.
• W2470461611 hasConcept C104317684 @default.
• W2470461611 hasConcept C123894998 @default.
• W2470461611 hasConcept C143065580 @default.
• W2470461611 hasConcept C147816474 @default.
• W2470461611 hasConcept C153911025 @default.
• W2470461611 hasConcept C16318435 @default.
• W2470461611 hasConcept C167625842 @default.
• W2470461611 hasConcept C181199279 @default.
• W2470461611 hasConcept C2779926675 @default.
• W2470461611 hasConcept C30417494 @default.
• W2470461611 hasConcept C40767141 @default.
• W2470461611 hasConcept C515207424 @default.
• W2470461611 hasConcept C55493867 @default.
• W2470461611 hasConcept C86803240 @default.
• W2470461611 hasConceptScore W2470461611C104317684 @default.
• W2470461611 hasConceptScore W2470461611C123894998 @default.
• W2470461611 hasConceptScore W2470461611C143065580 @default.
• W2470461611 hasConceptScore W2470461611C147816474 @default.
• W2470461611 hasConceptScore W2470461611C153911025 @default.
• W2470461611 hasConceptScore W2470461611C16318435 @default.
• W2470461611 hasConceptScore W2470461611C167625842 @default.
• W2470461611 hasConceptScore W2470461611C181199279 @default.
• W2470461611 hasConceptScore W2470461611C2779926675 @default.
• W2470461611 hasConceptScore W2470461611C30417494 @default.
• W2470461611 hasConceptScore W2470461611C40767141 @default.
• W2470461611 hasConceptScore W2470461611C515207424 @default.
• W2470461611 hasConceptScore W2470461611C55493867 @default.
• W2470461611 hasConceptScore W2470461611C86803240 @default.
• W2470461611 hasLocation W24704616111 @default.
• W2470461611 hasOpenAccess W2470461611 @default.
• W2470461611 hasPrimaryLocation W24704616111 @default.
• W2470461611 hasRelatedWork W1485999498 @default.
• W2470461611 hasRelatedWork W1607458008 @default.
• W2470461611 hasRelatedWork W1608151378 @default.
• W2470461611 hasRelatedWork W1646585585 @default.
• W2470461611 hasRelatedWork W1675708121 @default.
• W2470461611 hasRelatedWork W1989686473 @default.
• W2470461611 hasRelatedWork W1994334038 @default.
• W2470461611 hasRelatedWork W2010786308 @default.
• W2470461611 hasRelatedWork W2020933791 @default.
• W2470461611 hasRelatedWork W2027657428 @default.
• W2470461611 hasRelatedWork W2032904394 @default.
• W2470461611 hasRelatedWork W2036025776 @default.
• W2470461611 hasRelatedWork W2041808233 @default.
• W2470461611 hasRelatedWork W2056768891 @default.
• W2470461611 hasRelatedWork W2118032528 @default.
• W2470461611 hasRelatedWork W2405578899 @default.

• next