Matches in SemOpenAlex for { <https://semopenalex.org/work/W2470564585> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2470564585 abstract "UV light has often been used to induce the formation of covalent bonds between DNA (or RNA) and tightly-bound protein molecules. However, the internal photoreactions of nucleic acids and proteins limit the yield and complicate the analysis of intermolecular crosslinks. In an ongoing search for improved reaction specificity or new photoreactions in these systems, we have employed UV photons from a Nd:YAG-pumped dye laser and mid-IR photons from the Vanderbilt FEL. Having crosslinked several protein-nucleic acid systems with nanosecond UV laser pulses, we are currently studying the effect of various IR wavelengths on a model system (gene 32 protein and poly[dT]). We have found that irradiation with sufficiently intense FEL macropulses creates an altered form of gene 32 protein which was not observed with UV-only irradiation. The electrophoretic nobility of the product is consistent with the formation of a specific protein-protein crosslink. No evidence of the non-specific protein damage typically induced by UV light is found. The yield of the new photoproduct is apparently enhanced by exposure to FEL macropulses which are synchronized with UV laser pulses. With ideal exposure parameters, the two-color reaction effectively competes with UV-only reactions. Experiments designed to determine the reaction mechanism and to demonstrate FEL-inducedmore » reactions in other protein-nucleic acid systems are currently underway.« less" @default.
- W2470564585 created "2016-07-22" @default.
- W2470564585 creator A5008645195 @default.
- W2470564585 creator A5058452513 @default.
- W2470564585 date "1995-12-31" @default.
- W2470564585 modified "2023-09-27" @default.
- W2470564585 title "IR-UV photochemistry of protein-nucleic acid systems" @default.
- W2470564585 hasPublicationYear "1995" @default.
- W2470564585 type Work @default.
- W2470564585 sameAs 2470564585 @default.
- W2470564585 citedByCount "1" @default.
- W2470564585 crossrefType "journal-article" @default.
- W2470564585 hasAuthorship W2470564585A5008645195 @default.
- W2470564585 hasAuthorship W2470564585A5058452513 @default.
- W2470564585 hasConcept C111337013 @default.
- W2470564585 hasConcept C121332964 @default.
- W2470564585 hasConcept C12554922 @default.
- W2470564585 hasConcept C134121241 @default.
- W2470564585 hasConcept C178790620 @default.
- W2470564585 hasConcept C180577832 @default.
- W2470564585 hasConcept C185544564 @default.
- W2470564585 hasConcept C185592680 @default.
- W2470564585 hasConcept C191897082 @default.
- W2470564585 hasConcept C192562407 @default.
- W2470564585 hasConcept C24107716 @default.
- W2470564585 hasConcept C552990157 @default.
- W2470564585 hasConcept C55493867 @default.
- W2470564585 hasConcept C75473681 @default.
- W2470564585 hasConcept C86803240 @default.
- W2470564585 hasConceptScore W2470564585C111337013 @default.
- W2470564585 hasConceptScore W2470564585C121332964 @default.
- W2470564585 hasConceptScore W2470564585C12554922 @default.
- W2470564585 hasConceptScore W2470564585C134121241 @default.
- W2470564585 hasConceptScore W2470564585C178790620 @default.
- W2470564585 hasConceptScore W2470564585C180577832 @default.
- W2470564585 hasConceptScore W2470564585C185544564 @default.
- W2470564585 hasConceptScore W2470564585C185592680 @default.
- W2470564585 hasConceptScore W2470564585C191897082 @default.
- W2470564585 hasConceptScore W2470564585C192562407 @default.
- W2470564585 hasConceptScore W2470564585C24107716 @default.
- W2470564585 hasConceptScore W2470564585C552990157 @default.
- W2470564585 hasConceptScore W2470564585C55493867 @default.
- W2470564585 hasConceptScore W2470564585C75473681 @default.
- W2470564585 hasConceptScore W2470564585C86803240 @default.
- W2470564585 hasLocation W24705645851 @default.
- W2470564585 hasOpenAccess W2470564585 @default.
- W2470564585 hasPrimaryLocation W24705645851 @default.
- W2470564585 hasRelatedWork W1515847516 @default.
- W2470564585 hasRelatedWork W1654343933 @default.
- W2470564585 hasRelatedWork W1965639182 @default.
- W2470564585 hasRelatedWork W1986340243 @default.
- W2470564585 hasRelatedWork W1993312547 @default.
- W2470564585 hasRelatedWork W2005216471 @default.
- W2470564585 hasRelatedWork W2016350633 @default.
- W2470564585 hasRelatedWork W2017268977 @default.
- W2470564585 hasRelatedWork W2027790484 @default.
- W2470564585 hasRelatedWork W2028789715 @default.
- W2470564585 hasRelatedWork W2036575062 @default.
- W2470564585 hasRelatedWork W2046768117 @default.
- W2470564585 hasRelatedWork W2063131710 @default.
- W2470564585 hasRelatedWork W2092316037 @default.
- W2470564585 hasRelatedWork W236882799 @default.
- W2470564585 hasRelatedWork W2516466964 @default.
- W2470564585 hasRelatedWork W3008461088 @default.
- W2470564585 hasRelatedWork W3104046364 @default.
- W2470564585 hasRelatedWork W566919241 @default.
- W2470564585 hasRelatedWork W252200046 @default.
- W2470564585 isParatext "false" @default.
- W2470564585 isRetracted "false" @default.
- W2470564585 magId "2470564585" @default.
- W2470564585 workType "article" @default.