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- W2470827425 abstract "To establish the HPLC detection method for anatoxin-a in drinking water.1L water was collected, respectively, from raw and finished water in A and B water-plants provided source water by Qingcaosha Reservoir. Water samples were filtered by GF/C membrane, and then intracellular and dissolved anatoxin-a were enriched by weak cation-exchange (WCX) packing, respectively. The optimal ratio of trifluoroacetic acid (TFA) versus methanol for elution was determined. HPLC was employed for qualitative and quantitative analysis of anatoxin-a in water.Methanol containing 0.2% TFA had an optimal elution effect. The linear range of standard curve was 0.01-5 microg/ml and R2 value was 0.9999. The detection limit of anatoxin-a was 2.9 ng/L in water sample. The spiked recovery rates and the relative standard deviation (RSD%) were 75.2% - 100.6% and 6.38% - 8.76%, respectively. When detected continuously for six days, the average values were consistent with the true values, and RSD% of intra- and inter-day was among 1.60% - 8.90%. Anatoxin-a was detectable in raw water of both water-plants. 0.028 microg/L anatoxin-a of A water-plant and 0.137 microg/L anatoxin-a of B water-plant. Anatoxin-a was un-detectable in finished water of both water-plants.The current method can satisfy the detection of traces of anatoxin-a in drinking water and be used for continuous batch detection because of the low detection limit, high recovery rate and accuracy, good stability and reliability. More attention should be paid to surveillances of Qingcaosha Reservoir and raw and finished water from it, in order to prevent potential danger of Anatoxin-a on human health." @default.
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- W2470827425 date "2012-11-01" @default.
- W2470827425 modified "2023-10-03" @default.
- W2470827425 title "[Determination of anatoxin-a in raw and finished water from two water plants in Shanghai by high performance liquid chromatography]." @default.
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