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• W2470979746 abstract "Significance Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR associated (Cas) immunity relies on adaptive acquisition of spacers from foreign DNA into prokaryotes genomes. Efficient “primed” acquisition leads to selective intake of extra spacers from DNA molecules recognized by Cas proteins complexed with CRISPR RNAs partially matching foreign DNA. In contrast, fully matching targets are destroyed without new spacer acquisition. We show that when the rate of degradation of DNA with fully and partially matching targets is made equal, fully matching protospacers stimulate primed adaptation much more efficiently than partially matching ones. Thus, different outcomes of CRISPR-Cas response to two kinds of targets are caused by much more rapid destruction of fully matching targets rather than by structural differences in complexes formed on fully and partially matching targets." @default.
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• W2470979746 date "2016-06-20" @default.
• W2470979746 modified "2023-10-15" @default.
• W2470979746 title "Highly efficient primed spacer acquisition from targets destroyed by the <i>Escherichia coli</i> type I-E CRISPR-Cas interfering complex" @default.
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• W2470979746 doi "https://doi.org/10.1073/pnas.1602639113" @default.
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