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• W2471196099 abstract "Heartland virus (HRTV; Bunyaviridae: Phlebovirus ) is a recently described cause of human illness in the United States. After field studies conducted in 2012 implicated Amblyomma americanum (L.) as a vector of HRTV, we initiated experiments to assess the vector competence of A. americanum . Larval and nymphal ticks were immersed in high-titered suspensions of HRTV, and then tested for virus at various intervals postimmersion. In a later trial larval ticks were immersed in HRTV, followed by engorgement on a rabbit. A subset of postmolt nymphs was tested for HRTV to document transstadial transmission. Putatively infected nymphs were cofed with uninfected colony larvae to assess nonviremic transmission. In another trial, nymphs were fed on a rabbit and allowed to molt to the adult stage. Male and female ticks fed and mated upon a rabbit, and females were allowed to oviposit. Male and spent female ticks were tested for HRTV, and offspring of infected females were tested to assess vertical transmission. Infection rates of ≤50% were observed in immersed larvae and nymphs tested at intervals following immersion. Transstadial transmission from larvae to nymphs and then to adults was documented. HRTV was detected in a pool of nymphs molted from uninfected larvae cofed with infected nymphs. Vertical transmission of HRTV was observed in progeny of infected females. Infected females took longer to oviposit and produced fewer offspring. Serologic conversions (without viremia) in rabbits fed upon by immersed larvae or transstadially infected ticks indicate horizontal transmission of HRTV." @default.
• W2471196099 created "2016-07-22" @default.
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• W2471196099 date "2016-06-20" @default.
• W2471196099 modified "2023-09-30" @default.
• W2471196099 title "Transmission of Heartland Virus (Bunyaviridae:<i>Phlebovirus</i>) by Experimentally Infected<i>Amblyomma americanum</i>(Acari: Ixodidae)" @default.
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• W2471196099 doi "https://doi.org/10.1093/jme/tjw080" @default.
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