Matches in SemOpenAlex for { <https://semopenalex.org/work/W2471485102> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2471485102 endingPage "A81" @default.
- W2471485102 startingPage "A81.1" @default.
- W2471485102 abstract "<h3>Introduction</h3> Pancreatic cancer is one of the most common malignant tumours in the digestive tract in both sexes. Pancreatic adenocarcinoma shows the highest frequency of K-ras gene mutations among the common human cancers but the reasons are unknown. There is lack of knowledge of prevention, early diagnosis, and effective therapy. The molecular mechanism of pancreatic carcinogenesis remains to be fully explored from India. The study was designed to clarify the sensitivity and validity of K-ras point mutational analysis at codon 12 and codon 13 in North Indian patients with pancreatic diseases, and the possible correlation between the presence of mutation and histopathological findings. <h3>Methods</h3> Sixty-five North Indian patients with pancreatic ductal adenocarcinoma, and fifty chronic pancreatitis were enrolled in this study. Out of 65 pancreatic cancer, 24 patients were female (36.92%) and 41 patients were male (63%) having an average age of 47.42±11.32 years (range 22–70 years), 50 chronic pancreatitis only seven were female (14%) and 43 were male (86%) having an average age of 36.02±10.55 years (range 20–63 years). Codon 12 K-ras mutations were examined using the two step polymerase chain reaction (PCR) with RFLP, followed single-strand conformation polymorphism (SSCP) and automated DNA sequencing. <h3>Results</h3> The sensitivity of K-ras mutational analysis in surgically resected tissue samples of the pancreatic carcinoma was 72.30% (47/65) and mutations were found in 63% which was significantly higher than the chronic pancreatitis 33% (5/15) (P<0.01). The mutation pattern of K-ras codon 12 in pancreatic carcinoma 63% while it was 12% in chronic pancreatitis. The nucleotide sequences of K-ras showed the following transitions at codon12 GAG to GTG, GGC to GGG. The control group showed wild type GGA and GGC at codon12. It was also found that K-ras mutation rate was progressively increased from normal duct at the tumour-free resection margin to pancreatic carcinoma. <h3>Conclusion</h3> Overall, our findings support the observations that K-ras mutations play a key role in dysregulated growth of pancreatic cancer and may explain at least in part the aggressive behaviour of this tumour type." @default.
- W2471485102 created "2016-07-22" @default.
- W2471485102 creator A5033721173 @default.
- W2471485102 creator A5056972011 @default.
- W2471485102 creator A5075627594 @default.
- W2471485102 creator A5084451609 @default.
- W2471485102 creator A5089254788 @default.
- W2471485102 date "2010-04-01" @default.
- W2471485102 modified "2023-09-26" @default.
- W2471485102 title "PTU-079 K-ras gene mutations in pancreatic cancer and chronic pancreatitis patients in North Indian population" @default.
- W2471485102 doi "https://doi.org/10.1136/gut.2009.209072m" @default.
- W2471485102 hasPublicationYear "2010" @default.
- W2471485102 type Work @default.
- W2471485102 sameAs 2471485102 @default.
- W2471485102 citedByCount "0" @default.
- W2471485102 crossrefType "journal-article" @default.
- W2471485102 hasAuthorship W2471485102A5033721173 @default.
- W2471485102 hasAuthorship W2471485102A5056972011 @default.
- W2471485102 hasAuthorship W2471485102A5075627594 @default.
- W2471485102 hasAuthorship W2471485102A5084451609 @default.
- W2471485102 hasAuthorship W2471485102A5089254788 @default.
- W2471485102 hasConcept C104317684 @default.
- W2471485102 hasConcept C121608353 @default.
- W2471485102 hasConcept C126322002 @default.
- W2471485102 hasConcept C129219603 @default.
- W2471485102 hasConcept C142724271 @default.
- W2471485102 hasConcept C143998085 @default.
- W2471485102 hasConcept C144369459 @default.
- W2471485102 hasConcept C2775967933 @default.
- W2471485102 hasConcept C2776737053 @default.
- W2471485102 hasConcept C2778764654 @default.
- W2471485102 hasConcept C2780210213 @default.
- W2471485102 hasConcept C2781182431 @default.
- W2471485102 hasConcept C501734568 @default.
- W2471485102 hasConcept C54355233 @default.
- W2471485102 hasConcept C555283112 @default.
- W2471485102 hasConcept C71924100 @default.
- W2471485102 hasConcept C86803240 @default.
- W2471485102 hasConcept C90924648 @default.
- W2471485102 hasConceptScore W2471485102C104317684 @default.
- W2471485102 hasConceptScore W2471485102C121608353 @default.
- W2471485102 hasConceptScore W2471485102C126322002 @default.
- W2471485102 hasConceptScore W2471485102C129219603 @default.
- W2471485102 hasConceptScore W2471485102C142724271 @default.
- W2471485102 hasConceptScore W2471485102C143998085 @default.
- W2471485102 hasConceptScore W2471485102C144369459 @default.
- W2471485102 hasConceptScore W2471485102C2775967933 @default.
- W2471485102 hasConceptScore W2471485102C2776737053 @default.
- W2471485102 hasConceptScore W2471485102C2778764654 @default.
- W2471485102 hasConceptScore W2471485102C2780210213 @default.
- W2471485102 hasConceptScore W2471485102C2781182431 @default.
- W2471485102 hasConceptScore W2471485102C501734568 @default.
- W2471485102 hasConceptScore W2471485102C54355233 @default.
- W2471485102 hasConceptScore W2471485102C555283112 @default.
- W2471485102 hasConceptScore W2471485102C71924100 @default.
- W2471485102 hasConceptScore W2471485102C86803240 @default.
- W2471485102 hasConceptScore W2471485102C90924648 @default.
- W2471485102 hasIssue "Suppl 1" @default.
- W2471485102 hasLocation W24714851021 @default.
- W2471485102 hasOpenAccess W2471485102 @default.
- W2471485102 hasPrimaryLocation W24714851021 @default.
- W2471485102 hasRelatedWork W1899610622 @default.
- W2471485102 hasRelatedWork W1966674147 @default.
- W2471485102 hasRelatedWork W1979638928 @default.
- W2471485102 hasRelatedWork W1989955153 @default.
- W2471485102 hasRelatedWork W2054302145 @default.
- W2471485102 hasRelatedWork W2102245227 @default.
- W2471485102 hasRelatedWork W2152393478 @default.
- W2471485102 hasRelatedWork W2162346310 @default.
- W2471485102 hasRelatedWork W2976498758 @default.
- W2471485102 hasRelatedWork W4319232702 @default.
- W2471485102 hasVolume "59" @default.
- W2471485102 isParatext "false" @default.
- W2471485102 isRetracted "false" @default.
- W2471485102 magId "2471485102" @default.
- W2471485102 workType "article" @default.