Matches in SemOpenAlex for { <https://semopenalex.org/work/W2471661572> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2471661572 startingPage "266" @default.
- W2471661572 abstract "OBJECTIVE To investigate the effect of cancellous bone matrix gelatin (BMG) engineered with allogeneic chondrocytes in repairing articular cartilage defects in rabbits. METHODS Chondrocytes were seeded onto three-dimensional cancellous BMG and cultured in vitro for 12 days to prepare BMG-chondrocyte complexes. Under anesthesia with 2.5% pentobarbital sodium (1 ml/kg body weight), articular cartilage defects were made on the right knee joints of 38 healthy New Zealand white rabbits (regardless of sex, aged 4-5 months and weighing 2.5-3 kg) and the defects were then treated with 2.5% trypsin. Then BMG-chondrocyte complex (Group A, n=18), BMG (Group B, n=10), and nothing (Group C, n=10) were implanted into the cartilage defects, respectively. The repairing effects were assessed by macroscopic, histologic, transmission electron microscopic (TEM) observation, immunohistochemical examination and in situ hybridization detection, respectively, at 2, 4, 8, 12 and 24 weeks after operation. RESULTS Cancellous BMG was degraded within 8 weeks after operation. In Group A, lymphocyte infiltration was observed around the graft. At 24 weeks after operation, the cartilage defects were repaired by cartilage tissues and the articular cartilage and subchondral bone were soundly healed. Proteoglycan and type II collagen were detected in the matrix of the repaired tissues by Safranin-O staining and immunohistochemical staining, respectively. In situ hybridization proved gene expression of type II collagen in the cytoplasm of chondrocytes in the repaired tissues. TEM observation showed that chondrocytes and cartilage matrix in repaired tissues were almost same as those in the normal articular cartilage. In Group B, the defects were repaired by cartilage-fibrous tissues. In Group C, the defects were repaired only by fibrous tissues. CONCLUSIONS Cancellous BMG can be regarded as the natural cell scaffolds for cartilage tissue engineering. Articular cartilage defects can be repaired by cancellous BMG engineered with allogeneic chondrocytes. The nature of repaired tissues is closest to the normal cartilage. Local administration of trypsin can promote the adherence of repaired tissues to host tissues. Transplantation of allogeneic chondrocytes has immunogenicity, but the immune reaction is weak." @default.
- W2471661572 created "2016-07-22" @default.
- W2471661572 creator A5000262461 @default.
- W2471661572 creator A5002523610 @default.
- W2471661572 creator A5015102287 @default.
- W2471661572 creator A5042002301 @default.
- W2471661572 creator A5043428427 @default.
- W2471661572 creator A5072900429 @default.
- W2471661572 creator A5082053383 @default.
- W2471661572 date "2006-10-01" @default.
- W2471661572 modified "2023-09-27" @default.
- W2471661572 title "Repairing articular cartilage defects with tissue-engineering cartilage in rabbits." @default.
- W2471661572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17026857" @default.
- W2471661572 hasPublicationYear "2006" @default.
- W2471661572 type Work @default.
- W2471661572 sameAs 2471661572 @default.
- W2471661572 citedByCount "2" @default.
- W2471661572 countsByYear W24716615722020 @default.
- W2471661572 crossrefType "journal-article" @default.
- W2471661572 hasAuthorship W2471661572A5000262461 @default.
- W2471661572 hasAuthorship W2471661572A5002523610 @default.
- W2471661572 hasAuthorship W2471661572A5015102287 @default.
- W2471661572 hasAuthorship W2471661572A5042002301 @default.
- W2471661572 hasAuthorship W2471661572A5043428427 @default.
- W2471661572 hasAuthorship W2471661572A5072900429 @default.
- W2471661572 hasAuthorship W2471661572A5082053383 @default.
- W2471661572 hasConcept C104317684 @default.
- W2471661572 hasConcept C105702510 @default.
- W2471661572 hasConcept C106487976 @default.
- W2471661572 hasConcept C142724271 @default.
- W2471661572 hasConcept C150194340 @default.
- W2471661572 hasConcept C185592680 @default.
- W2471661572 hasConcept C189165786 @default.
- W2471661572 hasConcept C204232928 @default.
- W2471661572 hasConcept C2776350087 @default.
- W2471661572 hasConcept C2776832592 @default.
- W2471661572 hasConcept C2779335624 @default.
- W2471661572 hasConcept C2780550940 @default.
- W2471661572 hasConcept C2781013607 @default.
- W2471661572 hasConcept C2781403057 @default.
- W2471661572 hasConcept C43617362 @default.
- W2471661572 hasConcept C55493867 @default.
- W2471661572 hasConcept C71924100 @default.
- W2471661572 hasConcept C74864618 @default.
- W2471661572 hasConcept C81439078 @default.
- W2471661572 hasConceptScore W2471661572C104317684 @default.
- W2471661572 hasConceptScore W2471661572C105702510 @default.
- W2471661572 hasConceptScore W2471661572C106487976 @default.
- W2471661572 hasConceptScore W2471661572C142724271 @default.
- W2471661572 hasConceptScore W2471661572C150194340 @default.
- W2471661572 hasConceptScore W2471661572C185592680 @default.
- W2471661572 hasConceptScore W2471661572C189165786 @default.
- W2471661572 hasConceptScore W2471661572C204232928 @default.
- W2471661572 hasConceptScore W2471661572C2776350087 @default.
- W2471661572 hasConceptScore W2471661572C2776832592 @default.
- W2471661572 hasConceptScore W2471661572C2779335624 @default.
- W2471661572 hasConceptScore W2471661572C2780550940 @default.
- W2471661572 hasConceptScore W2471661572C2781013607 @default.
- W2471661572 hasConceptScore W2471661572C2781403057 @default.
- W2471661572 hasConceptScore W2471661572C43617362 @default.
- W2471661572 hasConceptScore W2471661572C55493867 @default.
- W2471661572 hasConceptScore W2471661572C71924100 @default.
- W2471661572 hasConceptScore W2471661572C74864618 @default.
- W2471661572 hasConceptScore W2471661572C81439078 @default.
- W2471661572 hasIssue "5" @default.
- W2471661572 hasLocation W24716615721 @default.
- W2471661572 hasLocation W24716615722 @default.
- W2471661572 hasOpenAccess W2471661572 @default.
- W2471661572 hasPrimaryLocation W24716615721 @default.
- W2471661572 hasRelatedWork W186585493 @default.
- W2471661572 hasRelatedWork W2034575194 @default.
- W2471661572 hasRelatedWork W2054604794 @default.
- W2471661572 hasRelatedWork W2347886384 @default.
- W2471661572 hasRelatedWork W2352266204 @default.
- W2471661572 hasRelatedWork W2353945950 @default.
- W2471661572 hasRelatedWork W2356782740 @default.
- W2471661572 hasRelatedWork W2357496693 @default.
- W2471661572 hasRelatedWork W2357794368 @default.
- W2471661572 hasRelatedWork W2360452785 @default.
- W2471661572 hasRelatedWork W2366680580 @default.
- W2471661572 hasRelatedWork W2374146040 @default.
- W2471661572 hasRelatedWork W2374252466 @default.
- W2471661572 hasRelatedWork W2376814708 @default.
- W2471661572 hasRelatedWork W2377210801 @default.
- W2471661572 hasRelatedWork W2387072882 @default.
- W2471661572 hasRelatedWork W2899001812 @default.
- W2471661572 hasRelatedWork W3030128741 @default.
- W2471661572 hasRelatedWork W3146635760 @default.
- W2471661572 hasRelatedWork W2587936346 @default.
- W2471661572 hasVolume "9" @default.
- W2471661572 isParatext "false" @default.
- W2471661572 isRetracted "false" @default.
- W2471661572 magId "2471661572" @default.
- W2471661572 workType "article" @default.