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• W2471958156 abstract "Event Abstract Back to Event Transplantation of fluorescent neural stem cells in healthy and stroke-affected mice Roland Pochet1*, Ivan Alic2, Nina Kosi2, Katarine Kapuralin3, Dunja Gorup2, Srecko Gajovic3 and Dinko Mitrecic2 1 Université Libre de Bruxelles, Faculté de Médecine, Belgium 2 Zagreb University School of Medicine, Croatian Institute for Brain Research, Croatia 3 Zagreb University School of Medicine, Croatian Institute for Brain Research, Croatia Transgenic mouse line Thy1-YFP selectively expressing fluorescent proteins in neurons has been developed to study many aspects of neuronal structure and connectivity. Taking advantage of the long term expression and repeated expression of Yellow Fluorescent Protein (YFP) labeled cells being minimally toxic and providing robust fluorescent signal (Feng et al.) we used YFP labeled stem cells. Neural stem cells were purified from the THY1 YFP-16 transgenic mouse line to study the birth and fate of transplanted YFP labelled neuronal stem cells in the healthy and stroke-affected mouse brain. During embryonic development THY1 – YFP positive cells were first observed at E12.5 and they were followed during differentiation of the nervous tissue. Thy1-positive cells were mostly present in prosencephalon, rombencephalon the spinal cord and in peripheral nerves of the embryo. Number of THY1 – YFP positive cells was 22% of the total cells and remained constant along the differentiation. Analysis on both RT-PCR and immunocytochemical level revealed that Thy1 positive cells during embryonic and in vitro differentiation were first nestin/SOX2 positive, which was gradually replaced by expression of MAP2, β3-tubulin and NeuN. Neural stem cells isolated from THY1 – YFP mouse strain transplanted in the striatum of the healthy and stroke affected mouse brain differentiated into mature neurons and were detectable even after 14 weeks, the end point of our experiment. Stroke region attracted transplanted cells but did not affect signal of Thy1. This study revealed that neural stem cells during in vitro differentiation and after transplantation in the brain followed the same pattern observed during development of embryo. SHORT COMMENT: En français: Il est aujourd'hui possible de transplanter des cellules souches et leurs dérivés (i.e. des cellules souches neurales). Cette nouvelle approche thérapeutique ouvre la voie vers le remplacement de neurones défectueux ou morts présents dans les maladies neurodégénératives. Ce concept doit être validé par la parfaite connaissance du devenir de ces cellules transplantées. Notre travail décrit le devenir de cellules souches neurales de souris une fois transplantées dans le cerveau de souris normales ou ayant subis un accident vasculaire cérébral. Le suivi de ces cellules a pu être réalisé grâce au marquage préalable de ces cellules par un marqueur fluorescent. Samenvatting in het Nederlands: Het is tegenwoordig mogelijk om stamcellen of de daarvan afgeleide cellen (bv. stamcellen van het zenuwstelsel) te transplanteren. Deze therapeutische benadering effent de weg naar het vervangen van gebrekkige of dode hersencellen bij degeneratieve hersenaandoeningen. Dit concept dient gevalideerd te worden door een gedegen kennis van het lot van zulke getransplanteerde cellen. Ons werk beschrijft het lot van neurale muizestamcellen na transplantatie in de hersenen van normale muizen of in de hersenen van muizen die een beroerte hebben opgelopen. Het opvolgen van deze cellen wordt mogelijk gemaakt door een voorafgaande markering met een fluorescerende merker. Figure 1 Acknowledgements This work has been supported by projects of the Croatian National Foundation (02.05/40), Foundation Adris and YoungBrain (EU-ESF) awarded to D.M., and FP7 Glowbrain project awarded to S.G. References Feng G1, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M, Nerbonne JM, Lichtman JW, Sanes JR. Neuron. 2000 Oct;28(1):41-51. Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Keywords: Neural Stem Cells, Yellow fluorescent protein, Transgenic mice, Stem Cell Transplantation, Stroke Conference: 6th Belgian Brain Congress, MONS, Belgium, 8 Oct - 8 Oct, 2016. Presentation Type: Poster Presentation Topic: Brain and brain diseases: between heredity and environment Citation: Pochet R, Alic I, Kosi N, Kapuralin K, Gorup D, Gajovic S and Mitrecic D (2016). Transplantation of fluorescent neural stem cells in healthy and stroke-affected mice. Conference Abstract: 6th Belgian Brain Congress. doi: 10.3389/conf.fnagi.2016.03.00002 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 14 Jun 2016; Published Online: 21 Jun 2016. * Correspondence: Prof. Roland Pochet, Université Libre de Bruxelles, Faculté de Médecine, Bruxelles, 1070, Belgium, rpochet@ulb.ac.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Roland Pochet Ivan Alic Nina Kosi Katarine Kapuralin Dunja Gorup Srecko Gajovic Dinko Mitrecic Google Roland Pochet Ivan Alic Nina Kosi Katarine Kapuralin Dunja Gorup Srecko Gajovic Dinko Mitrecic Google Scholar Roland Pochet Ivan Alic Nina Kosi Katarine Kapuralin Dunja Gorup Srecko Gajovic Dinko Mitrecic PubMed Roland Pochet Ivan Alic Nina Kosi Katarine Kapuralin Dunja Gorup Srecko Gajovic Dinko Mitrecic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2471958156 title "Transplantion of fluorescent neural stem cells in healthy and stroke-affected mice" @default.
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