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- W2472553589 abstract "This chapter outlines procedures for the expression of mammalian membrane-bound adenylyl cyclases (AC) in Sf9 cells and subsequent in vitro methods for assessing the activity of these cyclases. Membrane preparations derived from this overexpression system provide homogeneous sources of mammalian AC because AC that are endogenously expressed in Sf9 cells contribute low amounts of activity relative to the overexpressed enzyme. These approaches are applicable to all nine isoforms of mammalian membrane-bound AC isoforms." @default.
- W2472553589 created "2016-07-22" @default.
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- W2472553589 date "2003-11-15" @default.
- W2472553589 modified "2023-09-25" @default.
- W2472553589 title "Assays of Recombinant Adenylyl Cyclases Expressed in Sf9 Cells" @default.
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- W2472553589 doi "https://doi.org/10.1385/1-59259-430-1:77" @default.
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