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• W2473355380 abstract "The complex information-processing capabilities of the central nervous system emerge from intricate patterns of synaptic input-output relationships among various neuronal circuit components. Understanding these capabilities thus requires a precise description of the individual synapses that comprise neural networks. Recent advances in fluorescent protein engineering, along with developments in light-favoring tissue clearing and optical imaging techniques, have rendered light microscopy a potent candidate for large-scale analyses of synapses, their properties, and their connectivity. Optically imaging newly engineered fluorescent proteins tagged to synaptic proteins or microstructures enables the efficient, fine-resolution illumination of synaptic anatomy and function in large neural circuits. Here we review the latest progress in fluorescent protein-based molecular tools for imaging individual synapses and synaptic connectivity. We also identify associated technologies in gene delivery, tissue processing, and computational image analysis that will play a crucial role in bridging the gap between synapse- and system-level neuroscience." @default.
• W2473355380 created "2016-07-22" @default.
• W2473355380 creator A5019387083 @default.
• W2473355380 creator A5026656606 @default.
• W2473355380 creator A5058451627 @default.
• W2473355380 creator A5062064931 @default.
• W2473355380 date "2016-06-30" @default.
• W2473355380 modified "2023-09-28" @default.
• W2473355380 title "Advanced Fluorescence Protein-Based Synapse-Detectors" @default.
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• W2473355380 doi "https://doi.org/10.3389/fnsyn.2016.00016" @default.
• W2473355380 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4927625" @default.

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