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- W247379941 abstract "Wastewater generated during the manufacture of munitions often contains significant levels of nitrates and 2,4-dinitrotoluene (DNT). The objective of this project was to characterize the major biotransformation products formed from DNT under denitrifying conditions, and to identify the organisms responsible. In a denitrifying enrichment culture that used ethanol as the primary substrate, DNT was transformed primarily to 2-amino-4-nitrotoluene, 4-arnino-2-nitrotoluene, and 2,4-diaminotoluene. With extended incubation (>% 80 days), all of the 2,4-diaminotoluene subsequently disappeared. In cultures that received 14Cdnt, nearly all of the labeled metabolites remained in the aqueous phase. Approximately 35% consisted of insoluble material, while 29% was soluble hydrophobic and 32% was soluble hydrophilic. Two organisms were isolated from the enrichment: Pseudomonas aeruginosa and a much slower-growing rod. Pure cultures of P. aeruginosa only partially reduced DNT to 2,4-diaminotoluene under both aerobic and denitrifying conditions. Accumulation of 2-amino-4-nitrotoluene accounted for 25-45% of the DNT consumed, while 4-acetylamino-2-nitrotoluene accounted for 32-35%. Reduction and acetylation therefore appear to be major biotransformation pathways for DNT under both aerobic and denitrifying conditions." @default.
- W247379941 created "2016-06-24" @default.
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- W247379941 date "1995-04-10" @default.
- W247379941 modified "2023-09-26" @default.
- W247379941 title "Evaluation of biotransformation products from 2,4-dinitrotoluene under nitrate-reducing conditions. Final report" @default.
- W247379941 hasPublicationYear "1995" @default.
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