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- W2473863261 abstract "Background/Aims: To evaluate the antitumor immune response induced by truncated TERT (TERTt) mRNA transfected dendritic cells (DCs) in vivo. Methodology: Truncated mouse TERT sequence (according to mice telomerase reverse transcriptase mRNA 1776bp-2942bp) was cloned from B16 mice melanoma cells and inserted into pBluescript II KS(+) plasmid downstreaming of T7 promoter. The in vitro transcription was performed to prepare TERT t mRNA. The bone marrow-derived DCs isolated from BALB/c or C57B/L mice were electroporated with TERT t mRNA and recruited to immunize syngeneic naive mice respectively. The quantity and cytotoxic activity of tumor specific cytotoxic T lymphocytes (CTLs) in mice spleen were evaluated by using IFN-y enzyme-linked immunospot (ELIspot) and LDH release assay. The immunoprophylactic effects induced by TERT t mRNA transfected DC were evaluated in immunized-challenged mouse model. Results: TERT t was cloned and transcripted into TERT t mRNA in vitro. TERT t mRNA transfected bone marrow-derived DCs were prepared. As shown by transfecting with EGFP mRNA, the DC transfected efficiency is 35.1% and there was a subtle increase of costimulator and MHC-II molecule expression after electroporation. Immunization with TERT t mRNA transfected DCs can induce TERT t and TERT-specific IFN-y secreting CTLs in the spleen of immunized mice. The splenocytes isolated from mice immunized with TERT t mRNA transfected DCs showed specific cytotoxic activity against TERT t and TERT-positive target cells. Using a syngeneic cancer mouse model, it was shown that TERT t mRNA transfected DCs vaccination can suppress the growth of TERT-positive tumor inoculation. Conclusions: TERT t mRNA transfected bone marrow-derived DCs can evoke antitumor immune response in vivo effectively and TERT t can serve as a universal tumor associated antigen to produce DC-based tumor vaccine." @default.
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- W2473863261 date "2007-04-01" @default.
- W2473863261 modified "2023-09-27" @default.
- W2473863261 title "Truncated TERT mRNA transfected dendritic cells evoke TERT specific antitumor response in vivo." @default.
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