FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2474540495> ?p ?o ?g. }

• W2474540495 endingPage "e2195" @default.
• W2474540495 startingPage "e2195" @default.
• W2474540495 abstract "Background. Testicular ischemia reperfusion injury (tIRI) is considered the mechanism underlying the pathology of testicular torsion and detorsion. Left untreated, tIRI can induce testis dysfunction, damage to spermatogenesis and possible infertility. In this study, we aimed to assess the activities and expression of glycolytic enzymes (GEs) in the testis and their possible modulation during tIRI. The effect of fructose 1,6-diphosphate (FDP), a glycolytic intermediate, on tIRI was also investigated. Methods. Male Sprague-Dawley rats were divided into three groups: sham, unilateral tIRI, and tIRI + FDP (2 mg/kg). tIRI was induced by occlusion of the testicular artery for 1 h followed by 4 h of reperfusion. FDP was injected peritoneally 30 min prior to reperfusion. Histological and biochemical analyses were used to assess damage to spermatogenesis, activities of major GEs, and energy and oxidative stress markers. The relative mRNA expression of GEs was evaluated by real-time PCR. ELISA and immunohistochemistry were used to evaluate the expression of p53 and TP53-induced glycolysis and apoptosis regulator (TIGAR). Results. Histological analysis revealed tIRI-induced spermatogenic damage as represented by a significant decrease in the Johnsen biopsy score. In addition, tIRI reduced the activities of hexokinase 1, phosphofructokinase-1, glyceraldehyde 3-phosphate dehydrogenase, and lactate dehydrogenase C. However, mRNA expression downregulation was detected only for hexokinase 1, phosphoglycerate kinase 2, and lactate dehydrogenase C. ATP and NADPH depletion was also induced by tIRI and was accompanied by an increased Malondialdehyde concentration, reduced glutathione level, and reduced superoxide dismutase and catalase enzyme activities. The immunoexpression of p53 and TIGAR was markedly increased after tIRI. The above tIRI-induced alterations were attenuated by FDP treatment. Discussion. Our findings indicate that tIRI-induced spermatogenic damage is associated with dysregulation of GE activity and gene expression, which were associated with activation of the TIGAR/p53 pathway. FDP treatment had a beneficial effect on alleviating the damaging effects of tIRI. This study further emphasizes the importance of metabolic regulation for proper spermatogenesis." @default.
• W2474540495 created "2016-07-22" @default.
• W2474540495 creator A5040547021 @default.
• W2474540495 creator A5083191280 @default.
• W2474540495 date "2016-07-05" @default.
• W2474540495 modified "2023-10-14" @default.
• W2474540495 title "Altered expression profile of glycolytic enzymes during testicular ischemia reperfusion injury is associated with the p53/TIGAR pathway: effect of fructose 1,6-diphosphate" @default.
• W2474540495 cites W1499901308 @default.
• W2474540495 cites W1511003303 @default.
• W2474540495 cites W1569055690 @default.
• W2474540495 cites W1583426440 @default.
• W2474540495 cites W1707133078 @default.
• W2474540495 cites W1966320284 @default.
• W2474540495 cites W1978259246 @default.
• W2474540495 cites W1980443827 @default.
• W2474540495 cites W1980456818 @default.
• W2474540495 cites W1981905015 @default.
• W2474540495 cites W1990743716 @default.
• W2474540495 cites W1991069718 @default.
• W2474540495 cites W1992466559 @default.
• W2474540495 cites W2001930399 @default.
• W2474540495 cites W2003535478 @default.
• W2474540495 cites W2003815001 @default.
• W2474540495 cites W2008421111 @default.
• W2474540495 cites W2008710849 @default.
• W2474540495 cites W2011144569 @default.
• W2474540495 cites W2011250757 @default.
• W2474540495 cites W2013747211 @default.
• W2474540495 cites W2014192887 @default.
• W2474540495 cites W2017136163 @default.
• W2474540495 cites W2022894463 @default.
• W2474540495 cites W2025274017 @default.
• W2474540495 cites W2026031784 @default.
• W2474540495 cites W2026778985 @default.
• W2474540495 cites W2038293022 @default.
• W2474540495 cites W2042151892 @default.
• W2474540495 cites W2043964811 @default.
• W2474540495 cites W2045616407 @default.
• W2474540495 cites W2054547779 @default.
• W2474540495 cites W2063492525 @default.
• W2474540495 cites W2064465533 @default.
• W2474540495 cites W2065569779 @default.
• W2474540495 cites W2071018647 @default.
• W2474540495 cites W2076229947 @default.
• W2474540495 cites W2085280452 @default.
• W2474540495 cites W2094051883 @default.
• W2474540495 cites W2094380241 @default.
• W2474540495 cites W2103065406 @default.
• W2474540495 cites W2107277218 @default.
• W2474540495 cites W2108950836 @default.
• W2474540495 cites W2119239003 @default.
• W2474540495 cites W2119924772 @default.
• W2474540495 cites W2121391266 @default.
• W2474540495 cites W2130963659 @default.
• W2474540495 cites W2135719696 @default.
• W2474540495 cites W2137082142 @default.
• W2474540495 cites W2138329112 @default.
• W2474540495 cites W2144346537 @default.
• W2474540495 cites W2145352336 @default.
• W2474540495 cites W2157088514 @default.
• W2474540495 cites W2161126390 @default.
• W2474540495 cites W2163927084 @default.
• W2474540495 cites W2164229548 @default.
• W2474540495 cites W2167387657 @default.
• W2474540495 cites W2168531605 @default.
• W2474540495 cites W2192080449 @default.
• W2474540495 cites W2218766862 @default.
• W2474540495 cites W2231067246 @default.
• W2474540495 cites W2231781807 @default.
• W2474540495 cites W2254833392 @default.
• W2474540495 cites W2327272281 @default.
• W2474540495 cites W2332617654 @default.
• W2474540495 cites W2398056304 @default.
• W2474540495 cites W2467732724 @default.
• W2474540495 cites W40567873 @default.
• W2474540495 cites W615831052 @default.
• W2474540495 cites W833956060 @default.
• W2474540495 doi "https://doi.org/10.7717/peerj.2195" @default.
• W2474540495 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4941766" @default.
• W2474540495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27441124" @default.
• W2474540495 hasPublicationYear "2016" @default.
• W2474540495 type Work @default.
• W2474540495 sameAs 2474540495 @default.
• W2474540495 citedByCount "14" @default.
• W2474540495 countsByYear W24745404952017 @default.
• W2474540495 countsByYear W24745404952018 @default.
• W2474540495 countsByYear W24745404952019 @default.
• W2474540495 countsByYear W24745404952020 @default.
• W2474540495 countsByYear W24745404952021 @default.
• W2474540495 countsByYear W24745404952022 @default.
• W2474540495 countsByYear W24745404952023 @default.
• W2474540495 crossrefType "journal-article" @default.
• W2474540495 hasAuthorship W2474540495A5040547021 @default.
• W2474540495 hasAuthorship W2474540495A5083191280 @default.
• W2474540495 hasBestOaLocation W24745404951 @default.
• W2474540495 hasConcept C123765429 @default.
• W2474540495 hasConcept C126322002 @default.
• W2474540495 hasConcept C134018914 @default.

• next