Matches in SemOpenAlex for { <https://semopenalex.org/work/W2474642007> ?p ?o ?g. }
- W2474642007 endingPage "1456" @default.
- W2474642007 startingPage "1447" @default.
- W2474642007 abstract "The objective of the present study was to explore the in vitro and in vivo anticancer effects of Platycodin D (PD), derived from Platycodin grandiflorum, on highly metastatic MDA-MB-231 breast cancer cells. Using the MTT assay, we found that PD inhibited MDA-MB-231 cell growth in a concentration-dependent manner, with an IC50 value of 7.77±1.86 µM. Further studies showed that PD had anti-proliferative effects and induced cell cycle arrest in the G0/G1 phase. To explore the detailed mechanism(s) by which PD suppressed MDA-MB-231 cell growth, western blot analyses were used to detect the expression levels of proteins related to cell proliferation and survival. The data showed that PD decreased the expression of proteins related to the G0/G1 phases, downregulated the protein expression of MDM2, MDMX, and mutant p53, and increased the expression levels of p21 and p27 in vitro. We verified the effects of PD on the expression of MDM2, MDMX, mutant p53, p21 and p27 using a pcDNA3-Flag-MDM2 plasmid and MDM2 siRNA transfection, and found that PD inhibited MDA-MB-231 cell viability by targeting MDM2 and mutant p53. Compared with the corresponding parental cells, the cells with siRNA-MDM2 transfection had a greater decrease in cell viability and proliferation, while those with pcDNA3-MDM2 plasmid transfection did not show any increase in the effects of PD. We also established a MDA-MB-231 xenograft model in BALB/c nude mice, and found that PD significantly inhibited the growth of MDA-MB-231 xenograft tumors in these mice. The expression levels of various proteins in the tumor tissue exhibited changes similar to those observed in vitro. These findings indicate that PD exerted in vitro and in vivo anticancer effects against MDA-MB-231 breast cancer cells, that PD is a potential MDM2/MDMX inhibitor, and that the anticancer effects of PD were likely associated with its inhibition of these proteins. Our observations help to identify a mechanism by which PD functions as an anti-breast cancer agent." @default.
- W2474642007 created "2016-07-22" @default.
- W2474642007 creator A5005032895 @default.
- W2474642007 creator A5009570290 @default.
- W2474642007 creator A5017002887 @default.
- W2474642007 creator A5034935017 @default.
- W2474642007 creator A5047328298 @default.
- W2474642007 creator A5047933354 @default.
- W2474642007 creator A5053030535 @default.
- W2474642007 creator A5055330527 @default.
- W2474642007 creator A5063042333 @default.
- W2474642007 creator A5082807107 @default.
- W2474642007 creator A5085352453 @default.
- W2474642007 date "2016-07-13" @default.
- W2474642007 modified "2023-10-14" @default.
- W2474642007 title "Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 oncogene" @default.
- W2474642007 cites W1483633293 @default.
- W2474642007 cites W1576330810 @default.
- W2474642007 cites W1578014442 @default.
- W2474642007 cites W1854253459 @default.
- W2474642007 cites W1868102126 @default.
- W2474642007 cites W1964719993 @default.
- W2474642007 cites W1965535995 @default.
- W2474642007 cites W1966662013 @default.
- W2474642007 cites W1968075505 @default.
- W2474642007 cites W1981411106 @default.
- W2474642007 cites W1990037409 @default.
- W2474642007 cites W1991125047 @default.
- W2474642007 cites W1993358064 @default.
- W2474642007 cites W1993399844 @default.
- W2474642007 cites W2005288817 @default.
- W2474642007 cites W2005392579 @default.
- W2474642007 cites W2013312937 @default.
- W2474642007 cites W2015619266 @default.
- W2474642007 cites W2017462403 @default.
- W2474642007 cites W2032813428 @default.
- W2474642007 cites W2042316019 @default.
- W2474642007 cites W2063128372 @default.
- W2474642007 cites W2069452349 @default.
- W2474642007 cites W2073576377 @default.
- W2474642007 cites W2084831115 @default.
- W2474642007 cites W2092718140 @default.
- W2474642007 cites W2096268441 @default.
- W2474642007 cites W2101063496 @default.
- W2474642007 cites W2108918284 @default.
- W2474642007 cites W2109266176 @default.
- W2474642007 cites W2118534249 @default.
- W2474642007 cites W2123987844 @default.
- W2474642007 cites W2127932938 @default.
- W2474642007 cites W2129062251 @default.
- W2474642007 cites W2317236217 @default.
- W2474642007 cites W25654050 @default.
- W2474642007 cites W2917837889 @default.
- W2474642007 cites W4292477415 @default.
- W2474642007 doi "https://doi.org/10.3892/or.2016.4935" @default.
- W2474642007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27432230" @default.
- W2474642007 hasPublicationYear "2016" @default.
- W2474642007 type Work @default.
- W2474642007 sameAs 2474642007 @default.
- W2474642007 citedByCount "28" @default.
- W2474642007 countsByYear W24746420072017 @default.
- W2474642007 countsByYear W24746420072018 @default.
- W2474642007 countsByYear W24746420072019 @default.
- W2474642007 countsByYear W24746420072020 @default.
- W2474642007 countsByYear W24746420072021 @default.
- W2474642007 countsByYear W24746420072022 @default.
- W2474642007 countsByYear W24746420072023 @default.
- W2474642007 crossrefType "journal-article" @default.
- W2474642007 hasAuthorship W2474642007A5005032895 @default.
- W2474642007 hasAuthorship W2474642007A5009570290 @default.
- W2474642007 hasAuthorship W2474642007A5017002887 @default.
- W2474642007 hasAuthorship W2474642007A5034935017 @default.
- W2474642007 hasAuthorship W2474642007A5047328298 @default.
- W2474642007 hasAuthorship W2474642007A5047933354 @default.
- W2474642007 hasAuthorship W2474642007A5053030535 @default.
- W2474642007 hasAuthorship W2474642007A5055330527 @default.
- W2474642007 hasAuthorship W2474642007A5063042333 @default.
- W2474642007 hasAuthorship W2474642007A5082807107 @default.
- W2474642007 hasAuthorship W2474642007A5085352453 @default.
- W2474642007 hasBestOaLocation W24746420071 @default.
- W2474642007 hasConcept C1491633281 @default.
- W2474642007 hasConcept C150903083 @default.
- W2474642007 hasConcept C153911025 @default.
- W2474642007 hasConcept C185592680 @default.
- W2474642007 hasConcept C190283241 @default.
- W2474642007 hasConcept C207001950 @default.
- W2474642007 hasConcept C2776192174 @default.
- W2474642007 hasConcept C2780783641 @default.
- W2474642007 hasConcept C2781018059 @default.
- W2474642007 hasConcept C29537977 @default.
- W2474642007 hasConcept C502942594 @default.
- W2474642007 hasConcept C53227056 @default.
- W2474642007 hasConcept C54009773 @default.
- W2474642007 hasConcept C54355233 @default.
- W2474642007 hasConcept C55493867 @default.
- W2474642007 hasConcept C62112901 @default.
- W2474642007 hasConcept C81885089 @default.
- W2474642007 hasConcept C86803240 @default.