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• W2474667045 abstract "Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5–10 g), initial moisture (15–35 %), pH (4.0–6.0) and temperature (25–35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds−1 using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6.0, after 120 h of fermentation. The influence of PEG molar mass (1000–8000 g mol−1), phase concentrations (PEG 20.0–24.0 % w/w and sodium citrate 15–20 % w/w) and pH (6.0–8.0) on partition coefficient, purification factor, yield and selectivity of XY were determinate. Enzyme partitioning into the PEG rich phase was favored by M PEG 8000 (g mol−1), C PEG 24 % (w/w), C C 20 % (w/w) and pH 8.0, resulting in partition coefficient of 50.78, activity yield of 268 %, 7.20-fold purification factor and selectivity of 293. A. japonicus URM5620 has a potential role in the development of a bioprocess for the XY production using low-cost media. In addition, the present study proved it is feasible to extract xylanase from SSF by adopting the one step ATPS consisting of PEG/citrate." @default.
• W2474667045 created "2016-07-22" @default.
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• W2474667045 date "2016-06-23" @default.
• W2474667045 modified "2023-10-02" @default.
• W2474667045 title "Potential application of waste from castor bean (Ricinus communis L.) for production for xylanase of interest in the industry" @default.
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• W2474667045 doi "https://doi.org/10.1007/s13205-016-0463-1" @default.
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• W2474667045 hasPublicationYear "2016" @default.
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