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• W2474677321 abstract "Winged bean, Psophocarpus tetragonolobus (L.) DC., is similar to soybean in yield and nutritional value but more viable in tropical conditions. Here, we strengthen genetic resources for this orphan crop by producing a de novo transcriptome assembly and annotation of two Sri Lankan accessions (denoted herein as CPP34 [PI 491423] and CPP37 [PI 639033]), developing simple sequence repeat (SSR) markers, and identifying single nucleotide polymorphisms (SNPs) between geographically separated genotypes. A combined assembly based on 804,757 reads from two accessions produced 16,115 contigs with an N50 of 889 bp, over 90% of which has significant sequence similarity to other legumes. Combining contigs with singletons produced 97,241 transcripts. We identified 12,956 SSRs, including 2,594 repeats for which primers were designed and 5,190 high-confidence SNPs between Sri Lankan and Nigerian genotypes. The transcriptomic data sets generated here provide new resources for gene discovery and marker development in this orphan crop, and will be vital for future plant breeding efforts. We also analyzed the soybean trypsin inhibitor (STI) gene family, important plant defense genes, in the context of related legumes and found evidence for radiation of the Kunitz trypsin inhibitor (KTI) gene family within winged bean." @default.
• W2474677321 created "2016-07-22" @default.
• W2474677321 creator A5012242644 @default.
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• W2474677321 creator A5069724554 @default.
• W2474677321 creator A5073436151 @default.
• W2474677321 date "2016-06-30" @default.
• W2474677321 modified "2023-10-11" @default.
• W2474677321 title "Transcriptome sequencing and marker development in winged bean (Psophocarpus tetragonolobus; Leguminosae)" @default.
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• W2474677321 doi "https://doi.org/10.1038/srep29070" @default.
• W2474677321 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4928180" @default.
• W2474677321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27356763" @default.
• W2474677321 hasPublicationYear "2016" @default.
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