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- W2474817100 abstract "Telomere length is maintained in the ‘immortal’ germline yet evidence from mouse and human studies suggests that telomere length in oocytes is significantly shorter than in somatic tissues. We have proposed that telomere attrition in oocytes contributes to infertility in women, but the mechanisms underlying the effects of telomere length on reproduction in women are poorly understood. Telomere shortening arrests mitotic cells so we examined its effects during meiosis. Prospective observational study. Human germinal vesicle oocytes (GVs) were collected from patients at an academic fertility center and in-vitro matured for up to 48 hours following oocyte retrieval. Samples were assessed for polar body (PB) extrusion and germinal vesicle breakdown (GVBD) at 24 and 48 hours, then fixed for telomere length analysis. If no PB was observed at 48 hours, the sample was processed and staged as meiosis I (M1) or GV by absence or presence of GV nuclear structure respectively. Oocytes were denuded, treated with pronase and washed to exclude granulosa cells. One oocyte was delivered to each tube assuring no contamination with follicular cells. Telomere length was measured by single cell telomere pre-PCR amplification qPCR (SCT-pqPCR) and reported as telomere DNA (T) normalized to reference DNA (R) in a T/R ratio. T/R measures were log-transformed for statistical analyses to obtain a normal distribution. Mean telomere length of GV arrested oocytes (n = 35; 0.09 ± 0.44) was significantly less than that of oocytes that had matured to metaphase II (n = 74; 0.50 ± 0.54) oocytes (p < 0.001, paired t-test). Averaged per patient, mean telomere length of GV (n = 23; 0.00 ± 0.43) and M2 (n = 32; 0.50 ± 0.43) also differed significantly (p < 0.001). Telomere length of oocytes that had reached M1 by 48 hours was similar to that of M2 oocytes (n = 9; 0.28 ± 0.68; p =0.38). Arrested germinal vesicle oocytes exhibited less telomere DNA content than MI or MII oocytes. Telomere length influences progression through meiotic cell cycle, and this pilot study suggests oocyte telomere length may be an important regulator of oocyte maturation. Future studies will determine whether telomeres regulate mitotic and meiotic cell cycle via common regulatory pathways." @default.
- W2474817100 created "2016-07-22" @default.
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- W2474817100 date "2015-09-01" @default.
- W2474817100 modified "2023-10-16" @default.
- W2474817100 title "Telomere attrition in germinal vesicle arrested human oocytes" @default.
- W2474817100 doi "https://doi.org/10.1016/j.fertnstert.2015.07.617" @default.
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