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• W2474818302 abstract "Neuropeptides play a critical role in regulating animal reproduction. In vertebrates, GnRH, GnIH and kisspeptin are the key neuropeptide hormones of the reproductive axis, however, the reproductive axis for invertebrates is vague. Knowledge on ovarian development of the mud crab, Scylla paramamosain, is critical for aquaculture and resources management of the commercially important species. This study employed Illumina sequencing, reverse transcription-polymerase chain reaction and quantitative real-time PCR techniques to identify neuropeptides that may be involved in ovarian development of S. paramamosain. A total of 32 neuropeptide transcripts from two dozen neuropeptide families, 100 distinct mature peptides were predicted from the transcriptome data of female S. paramamosain cerebral ganglia. Among them, two families, i.e. GSEFLamide and WXXXRamide, were first identified from the cerebral ganglia of crustaceans. Of these neuropeptides, 21 transcripts of interest were selected for further confirmation and all of them were detected in the cerebral ganglia, as well as in other nervous tissues and the ovary. Most of them also had differential expression in the cerebral ganglia during various vitellogenic stages, suggesting their likely involvement in regulating vitellogenesis and ovarian maturation. Overall, these findings provide an important basis for subsequent studies on peptide function in reproduction of S. paramamosain." @default.
• W2474818302 created "2016-07-22" @default.
• W2474818302 creator A5018148913 @default.
• W2474818302 creator A5060327501 @default.
• W2474818302 creator A5061465638 @default.
• W2474818302 creator A5062944266 @default.
• W2474818302 date "2015-11-23" @default.
• W2474818302 modified "2023-09-29" @default.
• W2474818302 title "Neuropeptides in the cerebral ganglia of the mud crab, Scylla paramamosain: transcriptomic analysis and expression profiles during vitellogenesis" @default.
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• W2474818302 doi "https://doi.org/10.1038/srep17055" @default.
• W2474818302 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4655400" @default.
• W2474818302 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26592767" @default.
• W2474818302 hasPublicationYear "2015" @default.
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• W2474818302 hasAuthorship W2474818302A5061465638 @default.
• W2474818302 hasAuthorship W2474818302A5062944266 @default.

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