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- W2474831922 abstract "Rotavirus infection is the leading cause of severe diarrhea in infants and young children worldwide. The rotavirusnonstructuralproteinNSP4actsasaviralenterotoxin to induce diarrhea and causes Ca 21 -dependent transepithelial Cl 2 secretion in young mice. The cellular basis of this phenom- enonwasinvestigatedinaninvitrocelllinemodelforthehuman intestine. Intracellular calcium concentration ((Ca 21 )i) was monitored in fura-2-loaded HT-29 cells using microscope-based fluorescenceimaging.NSP4(1nMto5 mM)inducedbothCa 21 releasefromintracellularstoresandplasmalemmaCa 21 influx. DuringNSP4-induced(Ca 21 )imobilization,(Na 1 )ihomeostasis was not disrupted, demonstrating that NSP4 selectively regu- lated extracellular Ca 21 entry into these cells. The ED50of the NSP4 effect on peak (Ca 21 )i mobilization was 4.6 6 0.8 nM. Pretreatmentofcellswitheither2.3 310 23 unitsymltrypsinor 4.4 3 10 22 unitsyml chymotrypsin for 1-10 min abolished the NSP4-induced (Ca 21 )i mobilization. Superfusing cells with U-73122, an inhibitor of phospholipase C, ablated the NSP4 response.NSP4inducedarapidonsetandtransientstimulation ofinositol1,4,5-trisphosphate(IP3)productioninanIP3-specific radioreceptor assay. Taken together, these results suggest that NSP4 mobilizes (Ca 21 )i in human intestinal cells through re-" @default.
- W2474831922 created "2016-07-22" @default.
- W2474831922 creator A5012985372 @default.
- W2474831922 date "1997-01-01" @default.
- W2474831922 modified "2023-09-27" @default.
- W2474831922 title "The rotavirus enterotoxin NSP4 mobilizes intracellular calcium in human intestinal cells by stimulating phospholipase C-mediated inositol 1,4,5-trisphosphate production (calcium mobilizationydiarrheayHT-29 cellsyNSP4yrotavirus enterotoxin)" @default.
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