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• W2474832138 abstract "ABSTRACT Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from antibiotic efficacy studies, studies of growth under different nutritional or stress environments, to studies of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements." @default.
• W2474832138 created "2016-07-22" @default.
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• W2474832138 date "2016-07-04" @default.
• W2474832138 modified "2023-09-27" @default.
• W2474832138 title "General calibration of microbial growth in microplate readers" @default.
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• W2474832138 doi "https://doi.org/10.1101/061861" @default.
• W2474832138 hasPublicationYear "2016" @default.
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