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- W2474849134 abstract "Formation of micronuclei, induced by colchicine or colchemid, followed by the separation of micronuclei into microcells, is the only way to transfer intact chromosomes into mammalian cells. However, these chemicals show poor induction of micronuclei in plant cells, and therefore, microcell mediated chromosome transfer (MMCT) has received little attention in plants. Recently, we found that the herbicide Amiprophos-methyl (APM) induces micronuclei at a high frequency in cell suspension cultures of N.plumbaginifolia (1–3). In this paper we describe preliminary results of the development of microprotoplast mediated chromosome transfer, aiming at the stable transfer of a single donor chromosome into a recipient genome. This would open up ways to construct monochromosomal hybrid panels for gene mapping and expression studies." @default.
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- W2474849134 date "1988-01-01" @default.
- W2474849134 modified "2023-09-24" @default.
- W2474849134 title "Isolation of Microprotoplasts" @default.
- W2474849134 cites W1988548044 @default.
- W2474849134 doi "https://doi.org/10.1007/978-94-009-2788-9_105" @default.
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