Matches in SemOpenAlex for { <https://semopenalex.org/work/W2474880947> ?p ?o ?g. }
- W2474880947 abstract "Abstract Under hypertrophic stimulation, cardiomyocytes enter a hypermetabolic state and accelerate biomass accumulation. Although the molecular pathways that regulate protein levels are well-studied, the functional implications of RNA accumulation and its regulatory mechanisms in cardiomyocytes remain elusive. Here, we have elucidated the quantitative kinetics of RNA in cardiomyocytes through single cell imaging and c-Myc ( Myc )-mediated hypermetabolic analytical model using cultured cardiomyocytes. Nascent RNA labeling combined with single cell imaging demonstrated that Myc protein significantly increased the amount of global RNA production per cardiomyocyte. Chromatin immunoprecipitation with high-throughput sequencing clarified that overexpressed Myc bound to a specific set of genes and recruits RNA polymerase II. Among these genes, we identified Btg2 as a novel target of Myc. Btg2 overexpression significantly reduced cardiomyocyte surface area. Conversely, shRNA-mediated knockdown of Btg2 accelerated adrenergic stimulus-induced hypertrophy. Using mass spectrometry analysis, we determined that Btg2 binds a series of proteins that comprise mRNA deadenylation complexes. Intriguingly, Btg2 specifically suppresses cytosolic, but not nuclear, RNA levels. Btg2 knockdown further enhances cytosolic RNA accumulation in cardiomyocytes under adrenergic stimulation, suggesting that Btg2 negatively regulates reactive hypertrophy by negatively regulating RNA accumulation. Our findings provide insight into the functional significance of the mechanisms regulating RNA levels in cardiomyocytes." @default.
- W2474880947 created "2016-07-22" @default.
- W2474880947 creator A5015886646 @default.
- W2474880947 creator A5025504870 @default.
- W2474880947 creator A5027791632 @default.
- W2474880947 creator A5033043462 @default.
- W2474880947 creator A5042667479 @default.
- W2474880947 creator A5049340312 @default.
- W2474880947 creator A5065031449 @default.
- W2474880947 creator A5066544282 @default.
- W2474880947 creator A5067372215 @default.
- W2474880947 creator A5069082358 @default.
- W2474880947 creator A5070603586 @default.
- W2474880947 creator A5073523190 @default.
- W2474880947 creator A5076351619 @default.
- W2474880947 creator A5078668392 @default.
- W2474880947 creator A5080293775 @default.
- W2474880947 creator A5083418135 @default.
- W2474880947 date "2016-06-27" @default.
- W2474880947 modified "2023-10-06" @default.
- W2474880947 title "Btg2 is a Negative Regulator of Cardiomyocyte Hypertrophy through a Decrease in Cytosolic RNA" @default.
- W2474880947 cites W1518221901 @default.
- W2474880947 cites W1794800624 @default.
- W2474880947 cites W1927746554 @default.
- W2474880947 cites W1967444021 @default.
- W2474880947 cites W1976014395 @default.
- W2474880947 cites W1980093005 @default.
- W2474880947 cites W1980748010 @default.
- W2474880947 cites W1987261387 @default.
- W2474880947 cites W1991807334 @default.
- W2474880947 cites W1993807435 @default.
- W2474880947 cites W1996757904 @default.
- W2474880947 cites W2000134469 @default.
- W2474880947 cites W2004071564 @default.
- W2474880947 cites W2005133525 @default.
- W2474880947 cites W2007080030 @default.
- W2474880947 cites W2012036591 @default.
- W2474880947 cites W2018202368 @default.
- W2474880947 cites W2021536610 @default.
- W2474880947 cites W2022832110 @default.
- W2474880947 cites W2028675417 @default.
- W2474880947 cites W2028761750 @default.
- W2474880947 cites W2030892722 @default.
- W2474880947 cites W2033275544 @default.
- W2474880947 cites W2039357236 @default.
- W2474880947 cites W2051508016 @default.
- W2474880947 cites W2055477527 @default.
- W2474880947 cites W2056644877 @default.
- W2474880947 cites W2068575090 @default.
- W2474880947 cites W2077813547 @default.
- W2474880947 cites W2080758805 @default.
- W2474880947 cites W2081141657 @default.
- W2474880947 cites W2082017602 @default.
- W2474880947 cites W2085206746 @default.
- W2474880947 cites W2091154312 @default.
- W2474880947 cites W2091544975 @default.
- W2474880947 cites W2091998897 @default.
- W2474880947 cites W2094972443 @default.
- W2474880947 cites W2098589071 @default.
- W2474880947 cites W2112853435 @default.
- W2474880947 cites W2114818885 @default.
- W2474880947 cites W2122693759 @default.
- W2474880947 cites W2122925166 @default.
- W2474880947 cites W2124985265 @default.
- W2474880947 cites W2127181560 @default.
- W2474880947 cites W2128377517 @default.
- W2474880947 cites W2128859075 @default.
- W2474880947 cites W2129174269 @default.
- W2474880947 cites W2134013131 @default.
- W2474880947 cites W2135229538 @default.
- W2474880947 cites W2136817553 @default.
- W2474880947 cites W2138366746 @default.
- W2474880947 cites W2147138108 @default.
- W2474880947 cites W2148021854 @default.
- W2474880947 cites W2152720366 @default.
- W2474880947 cites W2154924534 @default.
- W2474880947 cites W2158217645 @default.
- W2474880947 cites W2161577298 @default.
- W2474880947 cites W2164416972 @default.
- W2474880947 cites W2167869412 @default.
- W2474880947 cites W2171808845 @default.
- W2474880947 doi "https://doi.org/10.1038/srep28592" @default.
- W2474880947 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4921833" @default.
- W2474880947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27346836" @default.
- W2474880947 hasPublicationYear "2016" @default.
- W2474880947 type Work @default.
- W2474880947 sameAs 2474880947 @default.
- W2474880947 citedByCount "20" @default.
- W2474880947 countsByYear W24748809472017 @default.
- W2474880947 countsByYear W24748809472018 @default.
- W2474880947 countsByYear W24748809472019 @default.
- W2474880947 countsByYear W24748809472020 @default.
- W2474880947 countsByYear W24748809472021 @default.
- W2474880947 countsByYear W24748809472022 @default.
- W2474880947 countsByYear W24748809472023 @default.
- W2474880947 crossrefType "journal-article" @default.
- W2474880947 hasAuthorship W2474880947A5015886646 @default.
- W2474880947 hasAuthorship W2474880947A5025504870 @default.
- W2474880947 hasAuthorship W2474880947A5027791632 @default.
- W2474880947 hasAuthorship W2474880947A5033043462 @default.