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- W2475156981 abstract "The genus Rubus contains a family of type III polyketide synthases which show differences in structure and function. We have cloned and characterized five aromatic polyketide synthase genes from raspberries. All five genes contain an intron of varying length and have 1173 bp coding sequences, with the exception of one gene that consists of 1149 bp. Four of the five genes encode proteins with 391 amino acid residues with a calculated protein mass of 42 kDa, while one gene coded for a shorter protein consisting of 383 amino acids. Sequence comparison of the five polyketide synthase genes showed high similarity both at the DNA and protein levels. Differences in the coding region were found mainly in the flanking sequences. Analysis of the reaction products showed that PKS1 and PKS5 were chalcone synthases, PKS2 that differs in six amino acids from PKS1 is silent, PKS3 is a p-coumarate triacetic acid lactone synthase (CTAS) and PKS4 is a benzalacetone synthase (BAS). The structural variations and the architecture of these PKS genes and enzymes is discussed." @default.
- W2475156981 created "2016-07-22" @default.
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- W2475156981 date "2007-01-11" @default.
- W2475156981 modified "2023-09-26" @default.
- W2475156981 title "Expression and Function of Aromatic Polyketide Synthase Genes in Raspberries (Rubus idaeus sp.)" @default.
- W2475156981 doi "https://doi.org/10.1021/bk-2007-0955.ch009" @default.
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