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- W2475377300 abstract "Because of their remarkable proliferative capacity and differentiation potential, human pluripotent stem cells (hPSCs) may provide a cell source for cell replacement therapies, drug screening, and disease modeling. The use of hPSCs in these applications requires a scalable, reproducible and controlled process. An adherent cell culture using cell feeder layers has been traditionally employed for the growth of human embryonic stem cells (hESCs). The utilization of defined culture components allows for reagent standardization, consistent cultures, and reduces risks from undefined components or undetectable animal pathogens. We firstly demonstrated that hESCs could be maintained on Matrigel or laminin-coated plates in serum-free medium conditioned by mouse feeders. We later developed culture techniques using defined media. Cells maintained in these culture systems show stable karyotype and proliferation rates, express pluripotency markers and differentiate into all three germ layers. In this chapter, we present culture methods for hPSC cultures and an approach for their characterization." @default.
- W2475377300 created "2016-08-23" @default.
- W2475377300 creator A5010898686 @default.
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- W2475377300 date "2013-01-01" @default.
- W2475377300 modified "2023-09-28" @default.
- W2475377300 title "Feeder-Free and Defined Culture Conditions for hESC Growth" @default.
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- W2475377300 doi "https://doi.org/10.1016/b978-0-12-385942-6.00019-6" @default.
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