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• W2475642694 endingPage "44" @default.
• W2475642694 startingPage "21" @default.
• W2475642694 abstract "The development of gene technology rests on the foundations of molecular biology that begins with the discovery of the double helical structure of DNA and the breaking of the genetic code as the major milestones. This chapter starts, therefore, with a brief review of the molecular biological basis of gene technology. During the past 20 years, an immense expansion has taken place in the understanding of the structure, organization, and regulation of genes. This expansion has, to a large extent, been the result of the development of new methods to manipulate, multiply, and study DNA fragments. The same new methods have a number of important applications in human medicine and, gradually, also in veterinary medicine. The basic mechanism for DNA replication is simple and elegant. The practical execution of this process in the cells, however, involves a large number of proteins, including some additional enzymes. The core of the new gene manipulation techniques is that one can join two pieces of DNA to form a recombinant DNA molecule, which then is multiplied or cloned in a suitable host organism so that a sufficient amount of the piece of the desired DNA is obtained. From the bacteria, the plasmid and the foreign DNA piece can be isolated in such amounts that it is possible to study the DNA in the electron microscope, determine its base sequence, inject it in fertilized ova, or use it as a diagnostic probe to mention some important uses." @default.
• W2475642694 created "2016-08-23" @default.
• W2475642694 creator A5048156889 @default.
• W2475642694 date "1997-01-01" @default.
• W2475642694 modified "2023-09-27" @default.
• W2475642694 title "DNA Technology in Diagnosis, Breeding, and Therapy" @default.
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