FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2476102182> ?p ?o ?g. }

• W2476102182 abstract "We expect that PKC δ activation will attenuate PMA-induced PMN SO release, whereas PKC δ inhibition will augment the response in comparison to untreated controls. As expected, PKC δ+ attenuated PMN SO release up to 50%, whereas, rottlerin augmented the PMA response up to 96% compared to untreated PMA controls (both p 93±4% in all study groups suggesting that the effects of rottlerin, PKC δ-, and PKC δ+ on PMA induced SO release were not related to cell viability. The data suggest that PKC δ negatively regulates PMN NADPH oxidase SO release. PKC δis relatively ineffective in regulating PMN SO release compared to rottlerin. The data suggest that inhibiting the ATP binding site of PKC δ by rottlerin may be more effective than the inhibition of the translocation of PKC δ to PMN substrates such as NADPH oxidase. Preliminary data shows that superoxide dismutase (SOD) inhibited untreated PMA (30 nM, n=3) induced SO release by 95±9% and also attenuated PMA plus rottlerin (20 μM, n=3) induced SO release by 96±4%. Cell viability was 96±1% in SOD treated groups. These preliminary results suggests that the effects of rottlerin to augment the reduction of ferricytochrome c in the presence of PMA are related to the generation of SO release. Additional trials will be preformed to confirm the preliminary results. Further research will be aimed at determining the phosphorylation sites of p47phox by PKC δ. Results Isolation of PMNs Male Sprague-Dawley rats (350-400 g, Charles River), used as PMN donors, were anesthetized with 2.5% isoflurane and given a 16 ml intraperitoneal injection of 0.5% glycogen (Sigma Chemical) dissolved in PBS. Rats were reanesthetized with isoflurane 16-18 h later, and the PMNs were harvested by peritoneal lavage in 30 ml of 0.9% NaCl, as previously described [5,8]. Peritoneal lavage fluid was centrifuged at 200 x g for 10 min at 4°C. Then, the PMNs were washed in 20 ml PBS and centrifuged at 200 g for 10 min at 4° C. PMNs were resuspended in 2.5 mL PBS and density was calculated. The PMNs preparation were >90% pure and >95% viable according to microscopic analysis and 0.3 trypan blue exclusion, respectively. Measurement of SO release from Rat PMNs The SO release from PMNs was measured spectrophotometrically (model 260 Gilford, Nova Biotech; El Cajon, CA) by the reduction of ferricytochrome c [5,8]. The PMNs (5 x 106) were resuspended in 450 μl of PBS and incubated with ferricytochrome c (100 μM, Sigma Chemical) in a total volume of 900 μl PBS in the presence or absence of PKC δ activator (Myr-MRAAEDPM, MW=1130 g/mol, 2.5-20 μM, n=10-13), PKC δ inhibitor (Myr-SFNSYELGSL, MW=1326 g/mol, 1-20 μM, n=7-10) or rottlerin (MW=516 g/mol, 0.625-20 μM, n=8-12) for 15 min at 37°C in spectrophotometric cells. The PMNs were stimulated with 15 or 30 nM PMA (MW=616 g/mol, n=13-26) in a final reaction volume of 1.0 ml. Absorbance at 550 nm was measured every 30 sec for up to 420 sec for PMA and the change in absorbance (SO release) from PMNs was determined relative to time 0. Cell viability among all study groups was determined by 0.3% trypan blue exclusion at the end of SO release assay. Viable cells remained unstained and non-viable cells stained blue. Statistical Analysis All data in the text and figures are presented as means ± S.E.M. The data were analyzed by analysis of variance using post hoc analysis with Fishers PLSD test. Probability values of <0.05 are considered to be statistically significant." @default.
• W2476102182 created "2016-08-23" @default.
• W2476102182 creator A5007579766 @default.
• W2476102182 creator A5009628749 @default.
• W2476102182 creator A5009804147 @default.
• W2476102182 creator A5011752636 @default.
• W2476102182 creator A5026505300 @default.
• W2476102182 creator A5027624441 @default.
• W2476102182 creator A5056026746 @default.
• W2476102182 creator A5068290128 @default.
• W2476102182 creator A5071737620 @default.
• W2476102182 date "2016-01-01" @default.
• W2476102182 modified "2023-09-24" @default.
• W2476102182 title "Protein kinase C (PKC) delta activation negatively regulates phorbol 12-myristate 13-acetate (PMA) induced superoxide (SO) release in polymorphonuclear leukocytes (PMNs)" @default.
• W2476102182 cites W1969361543 @default.
• W2476102182 cites W2016184567 @default.
• W2476102182 cites W2054127059 @default.
• W2476102182 cites W2093881154 @default.
• W2476102182 cites W2100577932 @default.
• W2476102182 cites W2397350565 @default.
• W2476102182 cites W2403589230 @default.
• W2476102182 hasPublicationYear "2016" @default.
• W2476102182 type Work @default.
• W2476102182 sameAs 2476102182 @default.
• W2476102182 citedByCount "0" @default.
• W2476102182 crossrefType "journal-article" @default.
• W2476102182 hasAuthorship W2476102182A5007579766 @default.
• W2476102182 hasAuthorship W2476102182A5009628749 @default.
• W2476102182 hasAuthorship W2476102182A5009804147 @default.
• W2476102182 hasAuthorship W2476102182A5011752636 @default.
• W2476102182 hasAuthorship W2476102182A5026505300 @default.
• W2476102182 hasAuthorship W2476102182A5027624441 @default.
• W2476102182 hasAuthorship W2476102182A5056026746 @default.
• W2476102182 hasAuthorship W2476102182A5068290128 @default.
• W2476102182 hasAuthorship W2476102182A5071737620 @default.
• W2476102182 hasConcept C11960822 @default.
• W2476102182 hasConcept C153911025 @default.
• W2476102182 hasConcept C181199279 @default.
• W2476102182 hasConcept C185592680 @default.
• W2476102182 hasConcept C195794163 @default.
• W2476102182 hasConcept C2775838275 @default.
• W2476102182 hasConcept C2777314051 @default.
• W2476102182 hasConcept C2778769476 @default.
• W2476102182 hasConcept C2779719074 @default.
• W2476102182 hasConcept C2780043322 @default.
• W2476102182 hasConcept C2780795997 @default.
• W2476102182 hasConcept C2909761558 @default.
• W2476102182 hasConcept C48349386 @default.
• W2476102182 hasConcept C55493867 @default.
• W2476102182 hasConcept C86803240 @default.
• W2476102182 hasConcept C99971728 @default.
• W2476102182 hasConceptScore W2476102182C11960822 @default.
• W2476102182 hasConceptScore W2476102182C153911025 @default.
• W2476102182 hasConceptScore W2476102182C181199279 @default.
• W2476102182 hasConceptScore W2476102182C185592680 @default.
• W2476102182 hasConceptScore W2476102182C195794163 @default.
• W2476102182 hasConceptScore W2476102182C2775838275 @default.
• W2476102182 hasConceptScore W2476102182C2777314051 @default.
• W2476102182 hasConceptScore W2476102182C2778769476 @default.
• W2476102182 hasConceptScore W2476102182C2779719074 @default.
• W2476102182 hasConceptScore W2476102182C2780043322 @default.
• W2476102182 hasConceptScore W2476102182C2780795997 @default.
• W2476102182 hasConceptScore W2476102182C2909761558 @default.
• W2476102182 hasConceptScore W2476102182C48349386 @default.
• W2476102182 hasConceptScore W2476102182C55493867 @default.
• W2476102182 hasConceptScore W2476102182C86803240 @default.
• W2476102182 hasConceptScore W2476102182C99971728 @default.
• W2476102182 hasLocation W24761021821 @default.
• W2476102182 hasOpenAccess W2476102182 @default.
• W2476102182 hasPrimaryLocation W24761021821 @default.
• W2476102182 hasRelatedWork W1966356301 @default.
• W2476102182 hasRelatedWork W1973377951 @default.
• W2476102182 hasRelatedWork W198221815 @default.
• W2476102182 hasRelatedWork W2018571781 @default.
• W2476102182 hasRelatedWork W2032453325 @default.
• W2476102182 hasRelatedWork W2035460716 @default.
• W2476102182 hasRelatedWork W2041084482 @default.
• W2476102182 hasRelatedWork W2060976839 @default.
• W2476102182 hasRelatedWork W2063372229 @default.
• W2476102182 hasRelatedWork W2067023320 @default.
• W2476102182 hasRelatedWork W2082759239 @default.
• W2476102182 hasRelatedWork W2387924322 @default.
• W2476102182 hasRelatedWork W2405501714 @default.
• W2476102182 hasRelatedWork W2415560239 @default.
• W2476102182 hasRelatedWork W2429787944 @default.
• W2476102182 hasRelatedWork W2431006461 @default.
• W2476102182 hasRelatedWork W345963328 @default.
• W2476102182 hasRelatedWork W2048081107 @default.
• W2476102182 hasRelatedWork W2116947115 @default.
• W2476102182 hasRelatedWork W3148933892 @default.
• W2476102182 isParatext "false" @default.
• W2476102182 isRetracted "false" @default.
• W2476102182 magId "2476102182" @default.
• W2476102182 workType "article" @default.