Matches in SemOpenAlex for { <https://semopenalex.org/work/W2476442084> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W2476442084 endingPage "66" @default.
- W2476442084 startingPage "55" @default.
- W2476442084 abstract "Chemically synthesized oligonucleotides are powerful tools in the molecular biologist’s repertoire. This chapter describes the design of oligonucleotides and their applications, with particular reference to their use in the isolation and characterization of recombinant DNA clones. Examples of their use are taken either from the literature or from work carried out in our laboratory. Two specific areas are covered: the design of oligonucleotides, based on peptide sequence derived from a purified protein, for use in the isolation of clones from recombinant DNA libraries, and the design of consensus oligonucleotide probes, corresponding to conserved sequences in multigene families, for use in the isolation and characterization of such related genes. We do not cover the use of oligonucleotides for the localization of mRNA transcripts by in situ hybridization or for the analysis of mRNA steady-state levels by Northern blot analysis since this has been described elsewhere (,). A detailed discussion of all of the factors involved in the formation and melting of oligonucleotide/target DNA duplexes is beyond the scope of this treatise; for this, readers are referred to the work of Lathe (). However, formulae for the calculation of melting temperatures and the application of the base analog inosine are described in the Notes." @default.
- W2476442084 created "2016-08-23" @default.
- W2476442084 creator A5055924518 @default.
- W2476442084 creator A5060408632 @default.
- W2476442084 date "2003-11-14" @default.
- W2476442084 modified "2023-09-23" @default.
- W2476442084 title "The Design and Use of Oligonucleotides" @default.
- W2476442084 cites W1985867674 @default.
- W2476442084 cites W2030442353 @default.
- W2476442084 doi "https://doi.org/10.1385/0-89603-199-3:55" @default.
- W2476442084 hasPublicationYear "2003" @default.
- W2476442084 type Work @default.
- W2476442084 sameAs 2476442084 @default.
- W2476442084 citedByCount "1" @default.
- W2476442084 crossrefType "book-chapter" @default.
- W2476442084 hasAuthorship W2476442084A5055924518 @default.
- W2476442084 hasAuthorship W2476442084A5060408632 @default.
- W2476442084 hasConcept C104317684 @default.
- W2476442084 hasConcept C111824103 @default.
- W2476442084 hasConcept C129312508 @default.
- W2476442084 hasConcept C153911025 @default.
- W2476442084 hasConcept C3017666073 @default.
- W2476442084 hasConcept C40767141 @default.
- W2476442084 hasConcept C54871578 @default.
- W2476442084 hasConcept C552990157 @default.
- W2476442084 hasConcept C55493867 @default.
- W2476442084 hasConcept C70721500 @default.
- W2476442084 hasConcept C86803240 @default.
- W2476442084 hasConceptScore W2476442084C104317684 @default.
- W2476442084 hasConceptScore W2476442084C111824103 @default.
- W2476442084 hasConceptScore W2476442084C129312508 @default.
- W2476442084 hasConceptScore W2476442084C153911025 @default.
- W2476442084 hasConceptScore W2476442084C3017666073 @default.
- W2476442084 hasConceptScore W2476442084C40767141 @default.
- W2476442084 hasConceptScore W2476442084C54871578 @default.
- W2476442084 hasConceptScore W2476442084C552990157 @default.
- W2476442084 hasConceptScore W2476442084C55493867 @default.
- W2476442084 hasConceptScore W2476442084C70721500 @default.
- W2476442084 hasConceptScore W2476442084C86803240 @default.
- W2476442084 hasLocation W24764420841 @default.
- W2476442084 hasOpenAccess W2476442084 @default.
- W2476442084 hasPrimaryLocation W24764420841 @default.
- W2476442084 hasRelatedWork W1965872676 @default.
- W2476442084 hasRelatedWork W1996979233 @default.
- W2476442084 hasRelatedWork W2034036767 @default.
- W2476442084 hasRelatedWork W2042073917 @default.
- W2476442084 hasRelatedWork W2061755273 @default.
- W2476442084 hasRelatedWork W2088139568 @default.
- W2476442084 hasRelatedWork W2133608598 @default.
- W2476442084 hasRelatedWork W2141646155 @default.
- W2476442084 hasRelatedWork W2316226786 @default.
- W2476442084 hasRelatedWork W2397465696 @default.
- W2476442084 isParatext "false" @default.
- W2476442084 isRetracted "false" @default.
- W2476442084 magId "2476442084" @default.
- W2476442084 workType "book-chapter" @default.