FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2476532643> ?p ?o ?g. }

• W2476532643 endingPage "1824" @default.
• W2476532643 startingPage "1812" @default.
• W2476532643 abstract "Urea amidolyase (UAL) is a multifunctional biotin-dependent enzyme that contributes to both bacterial and fungal pathogenicity by catalyzing the ATP-dependent cleavage of urea into ammonia and CO2 . UAL is comprised of two enzymatic components: urea carboxylase (UC) and allophanate hydrolase (AH). These enzyme activities are encoded on separate but proximally related genes in prokaryotes while, in most fungi, they are encoded by a single gene that produces a fusion enzyme on a single polypeptide chain. It is unclear whether the UC and AH activities are connected through substrate channeling or other forms of direct communication. Here, we use multiple biochemical approaches to demonstrate that there is no substrate channeling or interdomain/intersubunit communication between UC and AH. Neither stable nor transient interactions can be detected between prokaryotic UC and AH and the catalytic efficiencies of UC and AH are independent of one another. Furthermore, an artificial fusion of UC and AH does not significantly alter the AH enzyme activity or catalytic efficiency. These results support the surprising functional independence of AH from UC in both the prokaryotic and fungal UAL enzymes and serve as an important reminder that the evolution of multifunctional enzymes through gene fusion events does not always correlate with enhanced catalytic function." @default.
• W2476532643 created "2016-08-23" @default.
• W2476532643 creator A5044930993 @default.
• W2476532643 creator A5072036411 @default.
• W2476532643 creator A5082355776 @default.
• W2476532643 date "2016-08-05" @default.
• W2476532643 modified "2023-10-15" @default.
• W2476532643 title "The urea carboxylase and allophanate hydrolase activities of urea amidolyase are functionally independent" @default.
• W2476532643 cites W1548629691 @default.
• W2476532643 cites W1762507425 @default.
• W2476532643 cites W1965277029 @default.
• W2476532643 cites W1967662844 @default.
• W2476532643 cites W1969460352 @default.
• W2476532643 cites W1972946979 @default.
• W2476532643 cites W1974918433 @default.
• W2476532643 cites W1974987740 @default.
• W2476532643 cites W1982143750 @default.
• W2476532643 cites W1984139328 @default.
• W2476532643 cites W1990265320 @default.
• W2476532643 cites W1992764122 @default.
• W2476532643 cites W1995686191 @default.
• W2476532643 cites W2000292603 @default.
• W2476532643 cites W2000434472 @default.
• W2476532643 cites W2005722517 @default.
• W2476532643 cites W2013999114 @default.
• W2476532643 cites W2018324686 @default.
• W2476532643 cites W2026189786 @default.
• W2476532643 cites W2028100864 @default.
• W2476532643 cites W2035610197 @default.
• W2476532643 cites W2039896511 @default.
• W2476532643 cites W2041840130 @default.
• W2476532643 cites W2044072288 @default.
• W2476532643 cites W2045053532 @default.
• W2476532643 cites W2055685566 @default.
• W2476532643 cites W2067961759 @default.
• W2476532643 cites W2068511482 @default.
• W2476532643 cites W2069518997 @default.
• W2476532643 cites W2073901055 @default.
• W2476532643 cites W2088820058 @default.
• W2476532643 cites W2092642545 @default.
• W2476532643 cites W2092749957 @default.
• W2476532643 cites W2093434319 @default.
• W2476532643 cites W2096628809 @default.
• W2476532643 cites W2099140890 @default.
• W2476532643 cites W2101193082 @default.
• W2476532643 cites W2110057754 @default.
• W2476532643 cites W2112553122 @default.
• W2476532643 cites W2116275559 @default.
• W2476532643 cites W2124560890 @default.
• W2476532643 cites W2143838747 @default.
• W2476532643 cites W2144678314 @default.
• W2476532643 cites W2149241317 @default.
• W2476532643 cites W2153375254 @default.
• W2476532643 cites W2170696850 @default.
• W2476532643 cites W42033468 @default.
• W2476532643 cites W4233408616 @default.
• W2476532643 cites W4250838544 @default.
• W2476532643 cites W4251532368 @default.
• W2476532643 doi "https://doi.org/10.1002/pro.2990" @default.
• W2476532643 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5029531" @default.
• W2476532643 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27452902" @default.
• W2476532643 hasPublicationYear "2016" @default.
• W2476532643 type Work @default.
• W2476532643 sameAs 2476532643 @default.
• W2476532643 citedByCount "9" @default.
• W2476532643 countsByYear W24765326432017 @default.
• W2476532643 countsByYear W24765326432018 @default.
• W2476532643 countsByYear W24765326432019 @default.
• W2476532643 countsByYear W24765326432020 @default.
• W2476532643 countsByYear W24765326432022 @default.
• W2476532643 crossrefType "journal-article" @default.
• W2476532643 hasAuthorship W2476532643A5044930993 @default.
• W2476532643 hasAuthorship W2476532643A5072036411 @default.
• W2476532643 hasAuthorship W2476532643A5082355776 @default.
• W2476532643 hasBestOaLocation W24765326431 @default.
• W2476532643 hasConcept C140137476 @default.
• W2476532643 hasConcept C181199279 @default.
• W2476532643 hasConcept C185592680 @default.
• W2476532643 hasConcept C2780120296 @default.
• W2476532643 hasConcept C2780365088 @default.
• W2476532643 hasConcept C55493867 @default.
• W2476532643 hasConcept C86803240 @default.
• W2476532643 hasConceptScore W2476532643C140137476 @default.
• W2476532643 hasConceptScore W2476532643C181199279 @default.
• W2476532643 hasConceptScore W2476532643C185592680 @default.
• W2476532643 hasConceptScore W2476532643C2780120296 @default.
• W2476532643 hasConceptScore W2476532643C2780365088 @default.
• W2476532643 hasConceptScore W2476532643C55493867 @default.
• W2476532643 hasConceptScore W2476532643C86803240 @default.
• W2476532643 hasFunder F4320337354 @default.
• W2476532643 hasIssue "10" @default.
• W2476532643 hasLocation W24765326431 @default.
• W2476532643 hasLocation W24765326432 @default.
• W2476532643 hasLocation W24765326433 @default.
• W2476532643 hasLocation W24765326434 @default.
• W2476532643 hasLocation W24765326435 @default.
• W2476532643 hasOpenAccess W2476532643 @default.
• W2476532643 hasPrimaryLocation W24765326431 @default.

• next