FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2476865334> ?p ?o ?g. }

• W2476865334 endingPage "5959" @default.
• W2476865334 startingPage "5951" @default.
• W2476865334 abstract "ABSTRACT The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we identified a putative MAA ( mys ) gene cluster in two New Zealand isolates of Scytonema cf. crispum (UCFS10 and UCFS15). Homology to “ Anabaena -type” mys clusters suggested that this cluster was likely to be involved in shinorine biosynthesis. Surprisingly, high-performance liquid chromatography analysis of S . cf. crispum cell extracts revealed a complex MAA profile, including shinorine, palythine-serine, and their hexose-bound variants. It was hypothesized that a short-chain dehydrogenase (UCFS15_00405) encoded by a gene adjacent to the S . cf. crispum mys cluster was responsible for the conversion of shinorine to palythine-serine. Heterologous expression of MysABCE and UCFS15_00405 in Escherichia coli resulted in the exclusive production of the parent compound shinorine. Taken together, these results suggest that shinorine biosynthesis in S . cf. crispum proceeds via an Anabaena -type mechanism and that the genes responsible for the production of other MAA analogues, including palythine-serine and glycosylated analogues, may be located elsewhere in the genome. IMPORTANCE Recently, New Zealand isolates of S . cf. crispum were linked to the production of paralytic shellfish toxins for the first time, but no other natural products from this species have been reported. Thus, the species was screened for important natural product biosynthesis. The mycosporine-like amino acids (MAAs) are among the strongest absorbers of UV radiation produced in nature. The identification of novel MAAs is important from a biotechnology perspective, as these molecules are able to be utilized as sunscreens. This study has identified two novel MAAs that have provided several new avenues of future research related to MAA genetics and biosynthesis. Further, we have revealed that the genetic basis of MAA biosynthesis may not be clustered on the genome. The identification of the genes responsible for MAA biosynthesis is vital for future genetic engineering." @default.
• W2476865334 created "2016-08-23" @default.
• W2476865334 creator A5003700284 @default.
• W2476865334 creator A5043924724 @default.
• W2476865334 creator A5048296964 @default.
• W2476865334 creator A5067181898 @default.
• W2476865334 creator A5071365561 @default.
• W2476865334 creator A5083937091 @default.
• W2476865334 date "2016-10-01" @default.
• W2476865334 modified "2023-10-16" @default.
• W2476865334 title "Comparative Profiling and Discovery of Novel Glycosylated Mycosporine-Like Amino Acids in Two Strains of the Cyanobacterium Scytonema cf. crispum" @default.
• W2476865334 cites W1499213386 @default.
• W2476865334 cites W1544184273 @default.
• W2476865334 cites W1759358249 @default.
• W2476865334 cites W1883938359 @default.
• W2476865334 cites W1908709110 @default.
• W2476865334 cites W1911882834 @default.
• W2476865334 cites W1913121021 @default.
• W2476865334 cites W1981849332 @default.
• W2476865334 cites W1985191630 @default.
• W2476865334 cites W1986034035 @default.
• W2476865334 cites W1991658011 @default.
• W2476865334 cites W2016819575 @default.
• W2476865334 cites W2018373680 @default.
• W2476865334 cites W2020011754 @default.
• W2476865334 cites W2031348086 @default.
• W2476865334 cites W2036233390 @default.
• W2476865334 cites W2039834829 @default.
• W2476865334 cites W2040069658 @default.
• W2476865334 cites W2044384227 @default.
• W2476865334 cites W2045799417 @default.
• W2476865334 cites W2050791756 @default.
• W2476865334 cites W2051625677 @default.
• W2476865334 cites W2055475172 @default.
• W2476865334 cites W2057204261 @default.
• W2476865334 cites W2058245466 @default.
• W2476865334 cites W2061512416 @default.
• W2476865334 cites W2062369787 @default.
• W2476865334 cites W2063449724 @default.
• W2476865334 cites W2063692577 @default.
• W2476865334 cites W2064688707 @default.
• W2476865334 cites W2065118400 @default.
• W2476865334 cites W2065847101 @default.
• W2476865334 cites W2078295368 @default.
• W2476865334 cites W2080860303 @default.
• W2476865334 cites W2089373202 @default.
• W2476865334 cites W2097105739 @default.
• W2476865334 cites W2106307872 @default.
• W2476865334 cites W2117543390 @default.
• W2476865334 cites W2133145773 @default.
• W2476865334 cites W2133568756 @default.
• W2476865334 cites W2142356607 @default.
• W2476865334 cites W2143568528 @default.
• W2476865334 cites W2150036901 @default.
• W2476865334 cites W2166015109 @default.
• W2476865334 cites W2337294995 @default.
• W2476865334 cites W2397497430 @default.
• W2476865334 doi "https://doi.org/10.1128/aem.01633-16" @default.
• W2476865334 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5038028" @default.
• W2476865334 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27474710" @default.
• W2476865334 hasPublicationYear "2016" @default.
• W2476865334 type Work @default.
• W2476865334 sameAs 2476865334 @default.
• W2476865334 citedByCount "41" @default.
• W2476865334 countsByYear W24768653342017 @default.
• W2476865334 countsByYear W24768653342018 @default.
• W2476865334 countsByYear W24768653342019 @default.
• W2476865334 countsByYear W24768653342020 @default.
• W2476865334 countsByYear W24768653342021 @default.
• W2476865334 countsByYear W24768653342022 @default.
• W2476865334 countsByYear W24768653342023 @default.
• W2476865334 crossrefType "journal-article" @default.
• W2476865334 hasAuthorship W2476865334A5003700284 @default.
• W2476865334 hasAuthorship W2476865334A5043924724 @default.
• W2476865334 hasAuthorship W2476865334A5048296964 @default.
• W2476865334 hasAuthorship W2476865334A5067181898 @default.
• W2476865334 hasAuthorship W2476865334A5071365561 @default.
• W2476865334 hasAuthorship W2476865334A5083937091 @default.
• W2476865334 hasBestOaLocation W24768653341 @default.
• W2476865334 hasConcept C104317684 @default.
• W2476865334 hasConcept C108305142 @default.
• W2476865334 hasConcept C181199279 @default.
• W2476865334 hasConcept C2776414213 @default.
• W2476865334 hasConcept C36857842 @default.
• W2476865334 hasConcept C40767141 @default.
• W2476865334 hasConcept C515207424 @default.
• W2476865334 hasConcept C553450214 @default.
• W2476865334 hasConcept C55493867 @default.
• W2476865334 hasConcept C86803240 @default.
• W2476865334 hasConceptScore W2476865334C104317684 @default.
• W2476865334 hasConceptScore W2476865334C108305142 @default.
• W2476865334 hasConceptScore W2476865334C181199279 @default.
• W2476865334 hasConceptScore W2476865334C2776414213 @default.
• W2476865334 hasConceptScore W2476865334C36857842 @default.
• W2476865334 hasConceptScore W2476865334C40767141 @default.
• W2476865334 hasConceptScore W2476865334C515207424 @default.
• W2476865334 hasConceptScore W2476865334C553450214 @default.
• W2476865334 hasConceptScore W2476865334C55493867 @default.

• next