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- W2477013300 abstract "This chapter explains how biological material can be preserved in the quasi in vivo state at very high spatial and temporal resolution for structure. The basis for the unique efficacy of electron probe X-ray microanalysis (EPXMA) in identifying the distribution of elements in tissues and cells, presumably close to the in vivo state, is the method known as cryopreservation. Cryopreservation, beginning with quick freezing, is imperative for most biological applications of EPXMA to permanently immobilize elements in a given specimen while preserving cell structure close to the in vivo state. To that end, it must be capable of eliminating or at least minimizing untoward ice crystal growth that causes cryoartifacts. The objective of quick freezing is to withdraw heat from the specimen at a very fast rate. The major factors to be considered toward that goal are the nature of the coolant and the geometry of the specimen with its holder. The coolant is either solid (for example, copper for impact) or liquid (for example, propane for immersion) and must have large heat capacity and high conductivity at very low temperatures." @default.
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- W2477013300 date "1999-01-01" @default.
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- W2477013300 title "Principles of tissue preparation for high-resolution quantitative X-ray microanalysis" @default.
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- W2477013300 doi "https://doi.org/10.1016/b978-012371020-8/50010-x" @default.
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