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• W2477386332 abstract "This chapter describes the beige mouse model of disseminated Mycobacterium avium complex (MAC) infection. Primary cultures of MAC (M. kansasii or other mycobacteria) to be used for infection are obtained from clinical isolates of HIV-infected patients with disseminated MAC infection. Same-sex mice of 5–7 weeks old are allowed to acclimate for one week in the facility before being used in an experiment. They are housed in microisolator units and are randomly distributed six to a group. The mice are infected intravenous via a caudal tail vein. Observing mice for signs of infection is an important evaluation tool. The infected mice may exhibit a hunched posture with or without difficulty ambulating. They may isolate themselves from the rest of the group in their cage and breathing may become labored, and shivering may be apparent. There may be evidence of lack of eating and/or drinking and signs of diarrhea may be evident, such as areas of increased wetness in the bedding, especially in the corners of the cage. Efficacy is evaluated relative to the reduction of viable organisms in the spleens and lungs of therapy groups in comparison to those in the spleens and lungs of the control (infected, but untreated) groups. Additional comparison should be made between the therapy groups and the group given a standard therapy such as clarithromycin or azithromycin." @default.
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• W2477386332 date "1999-01-01" @default.
• W2477386332 modified "2023-10-16" @default.
• W2477386332 title "Beige Mouse Model of Disseminated Mycobacterium avium Complex Infection" @default.
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• W2477386332 doi "https://doi.org/10.1016/b978-012775390-4/50175-5" @default.
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