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- W2477779766 abstract "incorporated certain I4C-labelled aminoacids that arenormally components oftyrothricin into material that wasextracted byethanol fromtheprecipitate formed byadding acid. Whenthis material wasseparated bypaperandsilica-gel thin-layer chromatography andpaper electrophoresis '4Cwaslocated inthose regions that also contained gramicidin andtyrocidine. Fromastudy oftheproperties ofthesystem responsible fortheincorporation it wasdeduced that non-tyrothricin materials werepresent. Itwasshownthat themethods normally usedtocharacterize tyrothricin donotadequately distinguish between tyrothricinandnon-tyrothricin materials. However, amethod forseparating these materials was devised. This involved elution withethanol fromcolumns ofacid alumina followed bygel filtration onSephadex LH-20withdimethylformamide-water solvent. Thebehaviour of gramicidin andtyrocidine ontheSephadex LH-20column wasexamined, anditwasconcluded that theseparation wasnotcaused simply bygelfiltration ofunassociated molecules. Also, tyrocidine molecules withdifferent aminoacid compositions seemed tohave different affinities fortheSephadex LH-20column." @default.
- W2477779766 created "2016-08-23" @default.
- W2477779766 creator A5052512686 @default.
- W2477779766 creator A5071083044 @default.
- W2477779766 date "1972-01-01" @default.
- W2477779766 modified "2023-09-27" @default.
- W2477779766 title "TheUseofAcidAlumina andSephadex LH-20fortheSeparation and Characterization ofEthanol-Soluble Peptides Produced byBacillus brevis" @default.
- W2477779766 hasPublicationYear "1972" @default.
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