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- W2478989444 abstract "Publisher Summary This study summarizes various methods employed to characterize and quantify biological materials from human sources used in translational and clinical research. The measurement of biological compounds in body fluids and tissues is a critical component of clinical research and represents an objective endpoint for many trials, especially those involving therapeutic interventions. Over the past decade, there have been significant technological advances made to characterize and quantify biological compounds from in vivo sources and many of these can be exploited in translational research. The purpose of this chapter is to provide an overview of selected methods that are available to the clinical researcher to assess biological compounds from human material. There are six technologies that are discussed briefly: immunoassays and immunochemistry, chromatographic methods that include high pressure liquid chromatography and gas chromatography, mass spectrometry, genomics (gene expression microarrays), proteomics, and metabolomics. Some of the methods, such as immunoassays (immunoassay methodologies represent the most frequently used approach to measure biological compounds in translational and clinical research), chromatography, and mass spectrometry, are significantly more mature technologies than are genomics, proteomics, and metabolomics. In addition, the three former methodologies not only provide qualitative data but also are quantitative as opposed to the three latter methods that are more semiquantitative or qualitative in nature." @default.
- W2478989444 created "2016-08-23" @default.
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- W2478989444 date "2009-01-01" @default.
- W2478989444 modified "2023-09-25" @default.
- W2478989444 title "Measurement of Biological Materials" @default.
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- W2478989444 doi "https://doi.org/10.1016/b978-0-12-373639-0.00005-4" @default.
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