FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2479285410> ?p ?o ?g. }

• W2479285410 endingPage "6119" @default.
• W2479285410 startingPage "6109" @default.
• W2479285410 abstract "ABSTRACT The discovery and exploitation of the prokaryotic adaptive immunity system based on clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins have revolutionized genetic engineering. CRISPR-Cas tools have enabled extensive genome editing as well as efficient modulation of the transcriptional program in a multitude of organisms. Progress in the development of genetic engineering tools for the genus Clostridium has lagged behind that of many other prokaryotes, presenting the CRISPR-Cas technology an opportunity to resolve a long-existing issue. Here, we applied the Streptococcus pyogenes type II CRISPR-Cas9 (SpCRISPR-Cas9) system for genome editing in Clostridium acetobutylicum DSM792. We further explored the utility of the SpCRISPR-Cas9 machinery for gene-specific transcriptional repression. For proof-of-concept demonstration, a plasmid-encoded fluorescent protein gene was used for transcriptional repression in C. acetobutylicum . Subsequently, we targeted the carbon catabolite repression (CCR) system of C. acetobutylicum through transcriptional repression of the hprK gene encoding HPr kinase/phosphorylase, leading to the coutilization of glucose and xylose, which are two abundant carbon sources from lignocellulosic feedstocks. Similar approaches based on SpCRISPR-Cas9 for genome editing and transcriptional repression were also demonstrated in Clostridium pasteurianum ATCC 6013. As such, this work lays a foundation for the derivation of clostridial strains for industrial purposes. IMPORTANCE After recognizing the industrial potential of Clostridium for decades, methods for the genetic manipulation of these anaerobic bacteria are still underdeveloped. This study reports the implementation of CRISPR-Cas technology for genome editing and transcriptional regulation in Clostridium acetobutylicum , which is arguably the most common industrial clostridial strain. The developed genetic tools enable simpler, more reliable, and more extensive derivation of C. acetobutylicum mutant strains for industrial purposes. Similar approaches were also demonstrated in Clostridium pasteurianum , another clostridial strain that is capable of utilizing glycerol as the carbon source for butanol fermentation, and therefore can be arguably applied in other clostridial strains." @default.
• W2479285410 created "2016-08-23" @default.
• W2479285410 creator A5001705178 @default.
• W2479285410 creator A5007568857 @default.
• W2479285410 creator A5057938283 @default.
• W2479285410 creator A5059914775 @default.
• W2479285410 creator A5088490452 @default.
• W2479285410 date "2016-10-15" @default.
• W2479285410 modified "2023-10-15" @default.
• W2479285410 title "Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium" @default.
• W2479285410 cites W1505823785 @default.
• W2479285410 cites W1580150127 @default.
• W2479285410 cites W1964987246 @default.
• W2479285410 cites W1970196890 @default.
• W2479285410 cites W1971439989 @default.
• W2479285410 cites W1975508390 @default.
• W2479285410 cites W1978707258 @default.
• W2479285410 cites W1988059350 @default.
• W2479285410 cites W1991383594 @default.
• W2479285410 cites W1993009001 @default.
• W2479285410 cites W1994145955 @default.
• W2479285410 cites W2000046950 @default.
• W2479285410 cites W2008016941 @default.
• W2479285410 cites W2017482443 @default.
• W2479285410 cites W2017721099 @default.
• W2479285410 cites W2019670881 @default.
• W2479285410 cites W2020086177 @default.
• W2479285410 cites W2022707517 @default.
• W2479285410 cites W2031351113 @default.
• W2479285410 cites W2034048377 @default.
• W2479285410 cites W2036449301 @default.
• W2479285410 cites W2045435533 @default.
• W2479285410 cites W2047821263 @default.
• W2479285410 cites W2050480410 @default.
• W2479285410 cites W2050730094 @default.
• W2479285410 cites W2056955163 @default.
• W2479285410 cites W2060573994 @default.
• W2479285410 cites W2060576378 @default.
• W2479285410 cites W2064815984 @default.
• W2479285410 cites W2066991387 @default.
• W2479285410 cites W2076243547 @default.
• W2479285410 cites W2078699438 @default.
• W2479285410 cites W2088816616 @default.
• W2479285410 cites W2090041076 @default.
• W2479285410 cites W2094304821 @default.
• W2479285410 cites W2094480378 @default.
• W2479285410 cites W2098508815 @default.
• W2479285410 cites W2099047627 @default.
• W2479285410 cites W2099065852 @default.
• W2479285410 cites W2104713605 @default.
• W2479285410 cites W2106120443 @default.
• W2479285410 cites W2106986485 @default.
• W2479285410 cites W2108131456 @default.
• W2479285410 cites W2109918485 @default.
• W2479285410 cites W2110146873 @default.
• W2479285410 cites W2115420193 @default.
• W2479285410 cites W2124431991 @default.
• W2479285410 cites W2131207988 @default.
• W2479285410 cites W2139103751 @default.
• W2479285410 cites W2143752263 @default.
• W2479285410 cites W2144779133 @default.
• W2479285410 cites W2146905668 @default.
• W2479285410 cites W2151208862 @default.
• W2479285410 cites W2151391555 @default.
• W2479285410 cites W2154317105 @default.
• W2479285410 cites W2154395280 @default.
• W2479285410 cites W2154568091 @default.
• W2479285410 cites W2154797402 @default.
• W2479285410 cites W2155208047 @default.
• W2479285410 cites W2158849520 @default.
• W2479285410 cites W2160051211 @default.
• W2479285410 cites W2163241233 @default.
• W2479285410 cites W2165848622 @default.
• W2479285410 cites W2169244146 @default.
• W2479285410 cites W2170039745 @default.
• W2479285410 cites W2171280873 @default.
• W2479285410 cites W2171327609 @default.
• W2479285410 cites W2171393262 @default.
• W2479285410 cites W2199695484 @default.
• W2479285410 cites W2235953112 @default.
• W2479285410 cites W2416874763 @default.
• W2479285410 cites W4244026361 @default.
• W2479285410 cites W917998901 @default.
• W2479285410 doi "https://doi.org/10.1128/aem.02128-16" @default.
• W2479285410 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5068152" @default.
• W2479285410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27496775" @default.
• W2479285410 hasPublicationYear "2016" @default.
• W2479285410 type Work @default.
• W2479285410 sameAs 2479285410 @default.
• W2479285410 citedByCount "58" @default.
• W2479285410 countsByYear W24792854102016 @default.
• W2479285410 countsByYear W24792854102017 @default.
• W2479285410 countsByYear W24792854102018 @default.
• W2479285410 countsByYear W24792854102019 @default.
• W2479285410 countsByYear W24792854102020 @default.
• W2479285410 countsByYear W24792854102021 @default.
• W2479285410 countsByYear W24792854102022 @default.
• W2479285410 countsByYear W24792854102023 @default.

• next