Matches in SemOpenAlex for { <https://semopenalex.org/work/W2479739616> ?p ?o ?g. }
- W2479739616 abstract "Aeromonas hydrophila is a Gram-negative opportunistic pathogen of fish and terrestrial animals. In humans, A. hydrophila mainly causes gastroenteritis, septicaemia and tissue infections. The mechanisms of infection, the main virulence factors and the host immune response triggered by A. hydrophila have been studied in detail using murine models and adult fish. However, the great limitation of studying adult animals is that the animal must be sacrificed and its tissues/organs extracted, which prevents the study of the infectious processes in the whole living animal. Zebrafish larvae are being used for the analysis of several infectious diseases, but their use for studying the pathogenesis of A. hydrophila has never been explored. The great advantage of zebrafish larvae is their transparency during the first week after fertilization, which allows detailed descriptions of the infectious processes using in vivo imaging techniques such as differential interferential contrast (DIC) and fluorescence microscopy. Moreover, the availability of fluorescent pathogens and transgenic reporter zebrafish lines expressing fluorescent immune cells, immune marker genes or cytokines/chemokines allows the host-pathogen interactions to be characterized. The present study explores the suitability of zebrafish larvae to study the pathogenesis of A. hydrophila and the interaction mechanisms between the bacterium and the innate immune responses through an infection model using different routes for infection. We used an early-embryo infection model at 3 days post-fertilization (dpf) through the microinjection of A. hydrophila into the duct of Cuvier, caudal vein, notochord or muscle and two bath infection models using 4 dpf healthy and injured larvae. The latter resembled the natural conditions under which A. hydrophila produces infectious diseases in animals. We compared the cellular processes after infection in each anatomical site by confocal fluorescence imaging and determined the implication of inflammatory immune genes by measuring gene expression by qPCR." @default.
- W2479739616 created "2016-08-23" @default.
- W2479739616 creator A5006562505 @default.
- W2479739616 creator A5035725666 @default.
- W2479739616 creator A5061485833 @default.
- W2479739616 creator A5055058873 @default.
- W2479739616 date "2016-08-04" @default.
- W2479739616 modified "2023-09-27" @default.
- W2479739616 title "Establishment of Infection Models in Zebrafish Larvae (Danio rerio) to Study the Pathogenesis of Aeromonas hydrophila" @default.
- W2479739616 cites W1499500424 @default.
- W2479739616 cites W1578394711 @default.
- W2479739616 cites W1615535881 @default.
- W2479739616 cites W1809734056 @default.
- W2479739616 cites W1891908296 @default.
- W2479739616 cites W1891961100 @default.
- W2479739616 cites W1908290270 @default.
- W2479739616 cites W1964855454 @default.
- W2479739616 cites W1965508264 @default.
- W2479739616 cites W1965538575 @default.
- W2479739616 cites W1968295892 @default.
- W2479739616 cites W1968896842 @default.
- W2479739616 cites W1977059167 @default.
- W2479739616 cites W1979813052 @default.
- W2479739616 cites W1980353886 @default.
- W2479739616 cites W1988630756 @default.
- W2479739616 cites W1990484548 @default.
- W2479739616 cites W1990951015 @default.
- W2479739616 cites W1995964943 @default.
- W2479739616 cites W1998778525 @default.
- W2479739616 cites W2006166489 @default.
- W2479739616 cites W2006821186 @default.
- W2479739616 cites W2011262200 @default.
- W2479739616 cites W2012216170 @default.
- W2479739616 cites W2012684928 @default.
- W2479739616 cites W2012889426 @default.
- W2479739616 cites W2014843809 @default.
- W2479739616 cites W2015556938 @default.
- W2479739616 cites W2016262714 @default.
- W2479739616 cites W2017762021 @default.
- W2479739616 cites W2019454757 @default.
- W2479739616 cites W2019597896 @default.
- W2479739616 cites W2019997079 @default.
- W2479739616 cites W2020179710 @default.
- W2479739616 cites W2020508727 @default.
- W2479739616 cites W2028793851 @default.
- W2479739616 cites W2032227357 @default.
- W2479739616 cites W2039443848 @default.
- W2479739616 cites W2039830769 @default.
- W2479739616 cites W2041591721 @default.
- W2479739616 cites W2043544091 @default.
- W2479739616 cites W2044046770 @default.
- W2479739616 cites W2044887439 @default.
- W2479739616 cites W2045881301 @default.
- W2479739616 cites W2048630989 @default.
- W2479739616 cites W2050697866 @default.
- W2479739616 cites W2052807883 @default.
- W2479739616 cites W2053889157 @default.
- W2479739616 cites W2055683064 @default.
- W2479739616 cites W2062941705 @default.
- W2479739616 cites W2068728476 @default.
- W2479739616 cites W2071723955 @default.
- W2479739616 cites W2072424528 @default.
- W2479739616 cites W2077024843 @default.
- W2479739616 cites W2083848481 @default.
- W2479739616 cites W2084272607 @default.
- W2479739616 cites W2085432374 @default.
- W2479739616 cites W2093178459 @default.
- W2479739616 cites W2093413508 @default.
- W2479739616 cites W2097254456 @default.
- W2479739616 cites W2098216187 @default.
- W2479739616 cites W2099452097 @default.
- W2479739616 cites W2099688077 @default.
- W2479739616 cites W2101499815 @default.
- W2479739616 cites W2101672858 @default.
- W2479739616 cites W2102122111 @default.
- W2479739616 cites W2104058719 @default.
- W2479739616 cites W2104145948 @default.
- W2479739616 cites W2106631144 @default.
- W2479739616 cites W2107231437 @default.
- W2479739616 cites W2108244474 @default.
- W2479739616 cites W2114057849 @default.
- W2479739616 cites W2122032324 @default.
- W2479739616 cites W2122244080 @default.
- W2479739616 cites W2123033342 @default.
- W2479739616 cites W2123679840 @default.
- W2479739616 cites W2124107294 @default.
- W2479739616 cites W2125349610 @default.
- W2479739616 cites W2127165900 @default.
- W2479739616 cites W2128263760 @default.
- W2479739616 cites W2129806535 @default.
- W2479739616 cites W2130917915 @default.
- W2479739616 cites W2133699055 @default.
- W2479739616 cites W2134690112 @default.
- W2479739616 cites W2136985950 @default.
- W2479739616 cites W2138694950 @default.
- W2479739616 cites W2141023795 @default.
- W2479739616 cites W2141166469 @default.
- W2479739616 cites W2143494655 @default.
- W2479739616 cites W2143540026 @default.
- W2479739616 cites W2145943628 @default.