Matches in SemOpenAlex for { <https://semopenalex.org/work/W2479751307> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2479751307 endingPage "40" @default.
- W2479751307 startingPage "333" @default.
- W2479751307 abstract "The ability to modify human hepatocytes genetically is an essential first step in the development of liver-directed ex vivo gene therapy for inherited metabolic disease. The purpose of these studies was to prove that the genome of human hepatocytes can be altered successfully to express foreign genetic material.Human hepatocytes were plated at 2 or 4 x 10(6) cells/10 cm Primaria (Falcon, Oxnard, Calif.) plates. Fresh virus from the amphotropic viral producer cell line BAG, containing the Escherichia coli beta-galactosidase gene lacZ, was placed directly onto hepatocyte cultures and quantitative analysis of cells staining positive for the lacZ gene was undertaken. In a different human liver, a variety of viruses from producer cell lines containing clones of the human low-density lipoprotein (LDL) receptor were plated directly on cultures of human hepatocytes, and gene transfer was demonstrated by increased uptake of fluorescent-labeled LDL.Beta-galactosidase production in hepatocytes was assayed histochemically with the chromogenic substrate X-gal. The highest percentage of cells staining positive for expression of enzyme was seen at 4 x 10(6) cells/plate (43.66% +/- 1.02% vs 27.99% +/- 2.31%). Gene transfer was also documented by the uptake of fluorescent-labeled LDL with a variety of different vectors containing the human LDL receptor.(1) Human hepatocytes can be cultured in vitro and are susceptible to retroviral infection, (2) functional gene transfer is demonstrated by intracellular function of foreign genes, and (3) the level of expression appears dependent on plating density. We conclude that human hepatocytes are suitable targets for genetic manipulation and may play an important role in human gene therapy trials." @default.
- W2479751307 created "2016-08-23" @default.
- W2479751307 creator A5012014874 @default.
- W2479751307 creator A5051160919 @default.
- W2479751307 creator A5090760885 @default.
- W2479751307 date "1992-08-01" @default.
- W2479751307 modified "2023-09-23" @default.
- W2479751307 title "Retroviral-mediated gene transfer in human hepatocytes." @default.
- W2479751307 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1641772" @default.
- W2479751307 hasPublicationYear "1992" @default.
- W2479751307 type Work @default.
- W2479751307 sameAs 2479751307 @default.
- W2479751307 citedByCount "7" @default.
- W2479751307 countsByYear W24797513072019 @default.
- W2479751307 crossrefType "journal-article" @default.
- W2479751307 hasAuthorship W2479751307A5012014874 @default.
- W2479751307 hasAuthorship W2479751307A5051160919 @default.
- W2479751307 hasAuthorship W2479751307A5090760885 @default.
- W2479751307 hasConcept C104317684 @default.
- W2479751307 hasConcept C111599444 @default.
- W2479751307 hasConcept C150194340 @default.
- W2479751307 hasConcept C153911025 @default.
- W2479751307 hasConcept C202751555 @default.
- W2479751307 hasConcept C2776200302 @default.
- W2479751307 hasConcept C32470452 @default.
- W2479751307 hasConcept C40767141 @default.
- W2479751307 hasConcept C54009773 @default.
- W2479751307 hasConcept C54355233 @default.
- W2479751307 hasConcept C55493867 @default.
- W2479751307 hasConcept C79879829 @default.
- W2479751307 hasConcept C81885089 @default.
- W2479751307 hasConcept C86803240 @default.
- W2479751307 hasConcept C95444343 @default.
- W2479751307 hasConceptScore W2479751307C104317684 @default.
- W2479751307 hasConceptScore W2479751307C111599444 @default.
- W2479751307 hasConceptScore W2479751307C150194340 @default.
- W2479751307 hasConceptScore W2479751307C153911025 @default.
- W2479751307 hasConceptScore W2479751307C202751555 @default.
- W2479751307 hasConceptScore W2479751307C2776200302 @default.
- W2479751307 hasConceptScore W2479751307C32470452 @default.
- W2479751307 hasConceptScore W2479751307C40767141 @default.
- W2479751307 hasConceptScore W2479751307C54009773 @default.
- W2479751307 hasConceptScore W2479751307C54355233 @default.
- W2479751307 hasConceptScore W2479751307C55493867 @default.
- W2479751307 hasConceptScore W2479751307C79879829 @default.
- W2479751307 hasConceptScore W2479751307C81885089 @default.
- W2479751307 hasConceptScore W2479751307C86803240 @default.
- W2479751307 hasConceptScore W2479751307C95444343 @default.
- W2479751307 hasIssue "2" @default.
- W2479751307 hasLocation W24797513071 @default.
- W2479751307 hasOpenAccess W2479751307 @default.
- W2479751307 hasPrimaryLocation W24797513071 @default.
- W2479751307 hasRelatedWork W2052545121 @default.
- W2479751307 hasRelatedWork W2364319975 @default.
- W2479751307 hasRelatedWork W2385060395 @default.
- W2479751307 hasRelatedWork W2388714568 @default.
- W2479751307 hasRelatedWork W2397489422 @default.
- W2479751307 hasRelatedWork W2419114221 @default.
- W2479751307 hasRelatedWork W2469765221 @default.
- W2479751307 hasRelatedWork W2567673922 @default.
- W2479751307 hasRelatedWork W2794985616 @default.
- W2479751307 hasRelatedWork W3142015144 @default.
- W2479751307 hasVolume "112" @default.
- W2479751307 isParatext "false" @default.
- W2479751307 isRetracted "false" @default.
- W2479751307 magId "2479751307" @default.
- W2479751307 workType "article" @default.