Matches in SemOpenAlex for { <https://semopenalex.org/work/W2480373817> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2480373817 abstract "The ability to spatially and temporally control gene expression during development is crucial for the elucidation of gene function in vivo. The use of RNA interference (RNAi)-based technologies in combination with oviparous animal models allows for efficient, precise gene silencing. We have developed approaches using RNAi in the chicken embryo to analyze gene function during neural tube development. Here we describe the construction of plasmids that direct the expression of one or two artificial microRNAs (miRNAs) to knock down endogenous target protein/s upon electroporation into the spinal cord. The miRNA cassette is directly linked to a fluorescent protein reporter, which allows the faithful visualization of transfected cells. Different promoters/enhancers drive transcript expression in genetically defined cell subpopulations in the neural tube. Mixing multiple RNAi vectors allows combinatorial knockdowns of two or more genes in different cell types, thus permitting the rapid analysis of complex cellular and molecular interactions." @default.
- W2480373817 created "2016-08-23" @default.
- W2480373817 creator A5037912688 @default.
- W2480373817 creator A5069013921 @default.
- W2480373817 date "2016-01-01" @default.
- W2480373817 modified "2023-09-27" @default.
- W2480373817 title "In Ovo Electroporation of miRNA Plasmids to Silence Genes in a Temporally and Spatially Controlled Manner" @default.
- W2480373817 cites W1979033941 @default.
- W2480373817 cites W1980315256 @default.
- W2480373817 cites W1986251916 @default.
- W2480373817 cites W1993000387 @default.
- W2480373817 cites W1993537286 @default.
- W2480373817 cites W2003730468 @default.
- W2480373817 cites W2005089193 @default.
- W2480373817 cites W2008478427 @default.
- W2480373817 cites W2030936530 @default.
- W2480373817 cites W2035966961 @default.
- W2480373817 cites W2037501742 @default.
- W2480373817 cites W2044662844 @default.
- W2480373817 cites W2060714410 @default.
- W2480373817 cites W2072383478 @default.
- W2480373817 cites W2079747084 @default.
- W2480373817 cites W2080129352 @default.
- W2480373817 cites W2080970248 @default.
- W2480373817 cites W2081487945 @default.
- W2480373817 cites W2090923280 @default.
- W2480373817 cites W2116661471 @default.
- W2480373817 cites W2118372012 @default.
- W2480373817 cites W2119675301 @default.
- W2480373817 cites W2129439713 @default.
- W2480373817 cites W2132592493 @default.
- W2480373817 cites W2145062003 @default.
- W2480373817 cites W2152219310 @default.
- W2480373817 cites W2159955926 @default.
- W2480373817 cites W2161193917 @default.
- W2480373817 cites W2169305400 @default.
- W2480373817 cites W2170784838 @default.
- W2480373817 cites W2171685320 @default.
- W2480373817 cites W2398202798 @default.
- W2480373817 cites W2418697169 @default.
- W2480373817 cites W2526827868 @default.
- W2480373817 doi "https://doi.org/10.1007/7657_2016_5" @default.
- W2480373817 hasPublicationYear "2016" @default.
- W2480373817 type Work @default.
- W2480373817 sameAs 2480373817 @default.
- W2480373817 citedByCount "0" @default.
- W2480373817 crossrefType "book-chapter" @default.
- W2480373817 hasAuthorship W2480373817A5037912688 @default.
- W2480373817 hasAuthorship W2480373817A5069013921 @default.
- W2480373817 hasConcept C104317684 @default.
- W2480373817 hasConcept C119056186 @default.
- W2480373817 hasConcept C145059251 @default.
- W2480373817 hasConcept C150194340 @default.
- W2480373817 hasConcept C166703698 @default.
- W2480373817 hasConcept C186852380 @default.
- W2480373817 hasConcept C54355233 @default.
- W2480373817 hasConcept C67705224 @default.
- W2480373817 hasConcept C70721500 @default.
- W2480373817 hasConcept C86803240 @default.
- W2480373817 hasConcept C95444343 @default.
- W2480373817 hasConceptScore W2480373817C104317684 @default.
- W2480373817 hasConceptScore W2480373817C119056186 @default.
- W2480373817 hasConceptScore W2480373817C145059251 @default.
- W2480373817 hasConceptScore W2480373817C150194340 @default.
- W2480373817 hasConceptScore W2480373817C166703698 @default.
- W2480373817 hasConceptScore W2480373817C186852380 @default.
- W2480373817 hasConceptScore W2480373817C54355233 @default.
- W2480373817 hasConceptScore W2480373817C67705224 @default.
- W2480373817 hasConceptScore W2480373817C70721500 @default.
- W2480373817 hasConceptScore W2480373817C86803240 @default.
- W2480373817 hasConceptScore W2480373817C95444343 @default.
- W2480373817 hasLocation W24803738171 @default.
- W2480373817 hasOpenAccess W2480373817 @default.
- W2480373817 hasPrimaryLocation W24803738171 @default.
- W2480373817 hasRelatedWork W1927745891 @default.
- W2480373817 hasRelatedWork W1971276408 @default.
- W2480373817 hasRelatedWork W1979179112 @default.
- W2480373817 hasRelatedWork W1992755875 @default.
- W2480373817 hasRelatedWork W2007516069 @default.
- W2480373817 hasRelatedWork W2012118125 @default.
- W2480373817 hasRelatedWork W2014832324 @default.
- W2480373817 hasRelatedWork W2025579230 @default.
- W2480373817 hasRelatedWork W2030834300 @default.
- W2480373817 hasRelatedWork W2034824960 @default.
- W2480373817 hasRelatedWork W2060761159 @default.
- W2480373817 hasRelatedWork W2115613703 @default.
- W2480373817 hasRelatedWork W2245827401 @default.
- W2480373817 hasRelatedWork W2410215370 @default.
- W2480373817 hasRelatedWork W2593848117 @default.
- W2480373817 hasRelatedWork W2614874858 @default.
- W2480373817 hasRelatedWork W3135143003 @default.
- W2480373817 hasRelatedWork W3203538421 @default.
- W2480373817 hasRelatedWork W2623719484 @default.
- W2480373817 hasRelatedWork W3024470187 @default.
- W2480373817 isParatext "false" @default.
- W2480373817 isRetracted "false" @default.
- W2480373817 magId "2480373817" @default.
- W2480373817 workType "book-chapter" @default.