Matches in SemOpenAlex for { <https://semopenalex.org/work/W2480621975> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2480621975 endingPage "34" @default.
- W2480621975 startingPage "29" @default.
- W2480621975 abstract "This chapter describes methods that are commonly used in characterizing polycation-based gene delivery systems in in vitro transfection, focusing on complex formation between polycations and DNA. The degree of DNA condensation by polycations can be determined by an EtBr displacement assay using a fluorometer. The transformed cells are grown in larger quantities of Luria and Bertan (LB) broth. The plasmid DNA is isolated using the plasmid maxi kit from QIAgen according to the instructions of the manufacturer. The protocol can be used for all adherent cell types with slight modifications. For suspension type cells, cells need to be spun down before changing media. Tissue culture medium may vary, depending on the requirements of the cell line. The β-galactosidase activity in transfected cell lysates can be determined using the substrate Onitrophenyl-β-D-galactopyranoside (ONPG). Cytotoxicity of polycation/DNA complexes can be evaluated by the MTT assay. The assay is based on the cleavage of the yellow tetrazolium salt, MTT, to form dark blue formazan crystals by active mitochondria dehydrogenases." @default.
- W2480621975 created "2016-08-23" @default.
- W2480621975 creator A5000050008 @default.
- W2480621975 creator A5022679770 @default.
- W2480621975 creator A5029116662 @default.
- W2480621975 creator A5056562604 @default.
- W2480621975 creator A5090084419 @default.
- W2480621975 date "2006-01-01" @default.
- W2480621975 modified "2023-10-18" @default.
- W2480621975 title "Cationic Polymers for Gene DeliveryFormation of Polycation-DNA Complexes and in vitro Transfection" @default.
- W2480621975 cites W1576238436 @default.
- W2480621975 cites W1966390198 @default.
- W2480621975 cites W2021167060 @default.
- W2480621975 cites W2023104469 @default.
- W2480621975 cites W2024719999 @default.
- W2480621975 cites W2052917835 @default.
- W2480621975 cites W304669699 @default.
- W2480621975 doi "https://doi.org/10.1016/b978-012164730-8/50190-8" @default.
- W2480621975 hasPublicationYear "2006" @default.
- W2480621975 type Work @default.
- W2480621975 sameAs 2480621975 @default.
- W2480621975 citedByCount "0" @default.
- W2480621975 crossrefType "book-chapter" @default.
- W2480621975 hasAuthorship W2480621975A5000050008 @default.
- W2480621975 hasAuthorship W2480621975A5022679770 @default.
- W2480621975 hasAuthorship W2480621975A5029116662 @default.
- W2480621975 hasAuthorship W2480621975A5056562604 @default.
- W2480621975 hasAuthorship W2480621975A5090084419 @default.
- W2480621975 hasConcept C104317684 @default.
- W2480621975 hasConcept C109316439 @default.
- W2480621975 hasConcept C121332964 @default.
- W2480621975 hasConcept C153911025 @default.
- W2480621975 hasConcept C185592680 @default.
- W2480621975 hasConcept C202751555 @default.
- W2480621975 hasConcept C202800777 @default.
- W2480621975 hasConcept C22744801 @default.
- W2480621975 hasConcept C2776892249 @default.
- W2480621975 hasConcept C2780783641 @default.
- W2480621975 hasConcept C54009773 @default.
- W2480621975 hasConcept C54355233 @default.
- W2480621975 hasConcept C552990157 @default.
- W2480621975 hasConcept C55493867 @default.
- W2480621975 hasConcept C62520636 @default.
- W2480621975 hasConcept C81885089 @default.
- W2480621975 hasConcept C86803240 @default.
- W2480621975 hasConcept C91881484 @default.
- W2480621975 hasConceptScore W2480621975C104317684 @default.
- W2480621975 hasConceptScore W2480621975C109316439 @default.
- W2480621975 hasConceptScore W2480621975C121332964 @default.
- W2480621975 hasConceptScore W2480621975C153911025 @default.
- W2480621975 hasConceptScore W2480621975C185592680 @default.
- W2480621975 hasConceptScore W2480621975C202751555 @default.
- W2480621975 hasConceptScore W2480621975C202800777 @default.
- W2480621975 hasConceptScore W2480621975C22744801 @default.
- W2480621975 hasConceptScore W2480621975C2776892249 @default.
- W2480621975 hasConceptScore W2480621975C2780783641 @default.
- W2480621975 hasConceptScore W2480621975C54009773 @default.
- W2480621975 hasConceptScore W2480621975C54355233 @default.
- W2480621975 hasConceptScore W2480621975C552990157 @default.
- W2480621975 hasConceptScore W2480621975C55493867 @default.
- W2480621975 hasConceptScore W2480621975C62520636 @default.
- W2480621975 hasConceptScore W2480621975C81885089 @default.
- W2480621975 hasConceptScore W2480621975C86803240 @default.
- W2480621975 hasConceptScore W2480621975C91881484 @default.
- W2480621975 hasLocation W24806219751 @default.
- W2480621975 hasOpenAccess W2480621975 @default.
- W2480621975 hasPrimaryLocation W24806219751 @default.
- W2480621975 hasRelatedWork W1490746969 @default.
- W2480621975 hasRelatedWork W1995912941 @default.
- W2480621975 hasRelatedWork W1997892364 @default.
- W2480621975 hasRelatedWork W2036787356 @default.
- W2480621975 hasRelatedWork W2348326337 @default.
- W2480621975 hasRelatedWork W2364058230 @default.
- W2480621975 hasRelatedWork W2374321762 @default.
- W2480621975 hasRelatedWork W2377025740 @default.
- W2480621975 hasRelatedWork W2380962287 @default.
- W2480621975 hasRelatedWork W2471129735 @default.
- W2480621975 isParatext "false" @default.
- W2480621975 isRetracted "false" @default.
- W2480621975 magId "2480621975" @default.
- W2480621975 workType "book-chapter" @default.