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- W248236490 abstract "Chemical signaling has been found in the past to play a central role in bacterial biofilm formation, and may be important for the cyanobacterium Synechocystis sp. Strain PCC 6803. We investigate the cell-cell communication system of this species and whether it involves acyl homoserine lactone (AHL) signaling molecules. We use the biosensor, Chromobacterium violaceum CV026, which produces the violacein pigment in the presence of AHLs (McClean, et al., 1997). This biosensor was found to be a sensitive and quantitative method of detecting a variety of AHL chain lengths. Optimal growth temperature is 29°C and growth is not affected at low concentrations of acid, which is required for AHL stability. The physical structure of the biofilms of a super motile cyanobacterial mutant is also being analyzed using confocal microscopy. The biofilms begin as pillars 20–30um tall, grow to 50um deep and spread across the surface. The motile cyanobacterial cells exhibit unique biofilm formation and structure, and we are determining whether the process involves AHL signaling. This research is supported by the Wellesley College Biological Sciences Department and a Jerome A. Schiff Fellowship." @default.
- W248236490 created "2016-06-24" @default.
- W248236490 creator A5014184910 @default.
- W248236490 date "2013-04-01" @default.
- W248236490 modified "2023-09-27" @default.
- W248236490 title "Signaling Mechanisms and Physical Structure of Biofilm Growth in Synechocystis sp. Strain PCC 6803" @default.
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- W248236490 doi "https://doi.org/10.1096/fasebj.27.1_supplement.605.2" @default.
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