FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2482608553> ?p ?o ?g. }

• W2482608553 endingPage "854" @default.
• W2482608553 startingPage "847" @default.
• W2482608553 abstract "The Black and Tan Brachyury (BTBR) mouse strain is a valuable model for the study of long-term complications from obesity-induced type 2 diabetes mellitus and autism spectrum disorder. Due to technical difficulties with assisted reproduction, genetically modified animals on this background have previously been generated through extensive backcrossing, which is expensive and time-consuming. We successfully generated two separate transgenic mouse lines after direct zygote microinjection into this background strain. Additionally, we developed in vitro fertilization (IVF) methods for the BTBR mouse. We found low rates of fertilization and implantation in this strain, and identified the BTBR oocyte as the primary culprit of low success with BTBR IVF. We achieved an increase in live born pups from 5.9 to 35.6 % with IVF in the BTBR strain by use of BTBR females at a younger age (18–25 days), collection of oocytes 15–17 h after superovulation, and the use of supplemented fertilization media. This method eliminates the need for time consuming assisted embryo manipulations that are otherwise required for success with BTBR oocytes. This advancement provides an exciting opportunity to directly generate BTBR transgenics and gene-edited mice using both traditional and emerging genomic editing techniques, such as CRISPR/Cas9. These methods also allow effective colony preservation and rederivation with these strains. To our knowledge, this is the first report describing embryo manipulations in BTBR mice." @default.
• W2482608553 created "2016-08-23" @default.
• W2482608553 creator A5036050814 @default.
• W2482608553 creator A5077946474 @default.
• W2482608553 creator A5085239566 @default.
• W2482608553 creator A5086801368 @default.
• W2482608553 date "2016-08-11" @default.
• W2482608553 modified "2023-10-09" @default.
• W2482608553 title "Successful in vitro fertilization and generation of transgenics in Black and Tan Brachyury (BTBR) mice" @default.
• W2482608553 cites W1527740329 @default.
• W2482608553 cites W1572476794 @default.
• W2482608553 cites W1965388571 @default.
• W2482608553 cites W1968055555 @default.
• W2482608553 cites W1975543982 @default.
• W2482608553 cites W1979340145 @default.
• W2482608553 cites W1980891822 @default.
• W2482608553 cites W1998668351 @default.
• W2482608553 cites W2002783281 @default.
• W2482608553 cites W2006707889 @default.
• W2482608553 cites W2013997760 @default.
• W2482608553 cites W2014225402 @default.
• W2482608553 cites W2032739542 @default.
• W2482608553 cites W2038082979 @default.
• W2482608553 cites W2063771744 @default.
• W2482608553 cites W2064338721 @default.
• W2482608553 cites W2064857735 @default.
• W2482608553 cites W2071797996 @default.
• W2482608553 cites W2072907135 @default.
• W2482608553 cites W2075837574 @default.
• W2482608553 cites W2077012158 @default.
• W2482608553 cites W2085069626 @default.
• W2482608553 cites W2095887553 @default.
• W2482608553 cites W2116144339 @default.
• W2482608553 cites W2117642801 @default.
• W2482608553 cites W2117692196 @default.
• W2482608553 cites W2119124391 @default.
• W2482608553 cites W2128101266 @default.
• W2482608553 cites W2130688888 @default.
• W2482608553 cites W2131155467 @default.
• W2482608553 cites W2135862201 @default.
• W2482608553 cites W2143449370 @default.
• W2482608553 cites W2151825778 @default.
• W2482608553 cites W2154767575 @default.
• W2482608553 cites W2337962345 @default.
• W2482608553 cites W2343336146 @default.
• W2482608553 doi "https://doi.org/10.1007/s11248-016-9974-0" @default.
• W2482608553 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5106318" @default.
• W2482608553 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27515175" @default.
• W2482608553 hasPublicationYear "2016" @default.
• W2482608553 type Work @default.
• W2482608553 sameAs 2482608553 @default.
• W2482608553 citedByCount "8" @default.
• W2482608553 countsByYear W24826085532017 @default.
• W2482608553 countsByYear W24826085532019 @default.
• W2482608553 countsByYear W24826085532020 @default.
• W2482608553 countsByYear W24826085532021 @default.
• W2482608553 countsByYear W24826085532023 @default.
• W2482608553 crossrefType "journal-article" @default.
• W2482608553 hasAuthorship W2482608553A5036050814 @default.
• W2482608553 hasAuthorship W2482608553A5077946474 @default.
• W2482608553 hasAuthorship W2482608553A5085239566 @default.
• W2482608553 hasAuthorship W2482608553A5086801368 @default.
• W2482608553 hasBestOaLocation W24826085532 @default.
• W2482608553 hasConcept C104317684 @default.
• W2482608553 hasConcept C145103041 @default.
• W2482608553 hasConcept C16685009 @default.
• W2482608553 hasConcept C196843134 @default.
• W2482608553 hasConcept C2780915607 @default.
• W2482608553 hasConcept C28748434 @default.
• W2482608553 hasConcept C46122727 @default.
• W2482608553 hasConcept C512716672 @default.
• W2482608553 hasConcept C54355233 @default.
• W2482608553 hasConcept C71924100 @default.
• W2482608553 hasConcept C86803240 @default.
• W2482608553 hasConcept C87073359 @default.
• W2482608553 hasConceptScore W2482608553C104317684 @default.
• W2482608553 hasConceptScore W2482608553C145103041 @default.
• W2482608553 hasConceptScore W2482608553C16685009 @default.
• W2482608553 hasConceptScore W2482608553C196843134 @default.
• W2482608553 hasConceptScore W2482608553C2780915607 @default.
• W2482608553 hasConceptScore W2482608553C28748434 @default.
• W2482608553 hasConceptScore W2482608553C46122727 @default.
• W2482608553 hasConceptScore W2482608553C512716672 @default.
• W2482608553 hasConceptScore W2482608553C54355233 @default.
• W2482608553 hasConceptScore W2482608553C71924100 @default.
• W2482608553 hasConceptScore W2482608553C86803240 @default.
• W2482608553 hasConceptScore W2482608553C87073359 @default.
• W2482608553 hasFunder F4320332161 @default.
• W2482608553 hasFunder F4320337337 @default.
• W2482608553 hasFunder F4320337357 @default.
• W2482608553 hasIssue "6" @default.
• W2482608553 hasLocation W24826085531 @default.
• W2482608553 hasLocation W24826085532 @default.
• W2482608553 hasLocation W24826085533 @default.
• W2482608553 hasLocation W24826085534 @default.
• W2482608553 hasOpenAccess W2482608553 @default.
• W2482608553 hasPrimaryLocation W24826085531 @default.
• W2482608553 hasRelatedWork W1578843689 @default.

• next