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- W2483172008 abstract "1, 4-β-D-glucan cellobiohydrolases gene (cbh) was amplified from the genomic DNA of Trichoderma harzianum and T. reesei isolates which yielded a 500bp product. Purified PCR products were ligated to pGEMT cloning vector, transferred to E.coli DH5α separately and both the strands of the insert in this plasmid were sequenced. BLAST analysis of the cbh-1 sequences from Trichoderma species under study has three close homologues: cbh-1 of Hypocrea lixii, cbh-1 of A-25 strain of Hyocrea lixii. Sequence ID: gb|JN992645.1| and Sequence ID: gb|HM053612.1|, Hypocrea orientalis strain EU722 with Sequence ID: gb|JQ238604.1|. It also showed homology with conserved domain of Glycosyl Hydrolases family. Comparison of amino acid sequences of cbh-1 gene of both T. harzianum and T. reesei shows that the resultant sequence of T. harzianum cbh-1 can be translated to 133 amino acids and that from T. reesei has 124 amino acid sequences and has 75% sequence similarity. When compared with the T. viride homologs, it shows that there were 99.19 homolog similarity with T. reesei and 73.6 homolog similarity with T. harzianum." @default.
- W2483172008 created "2016-08-23" @default.
- W2483172008 creator A5063090075 @default.
- W2483172008 date "2013-01-01" @default.
- W2483172008 modified "2023-09-26" @default.
- W2483172008 title "Cloning, Characterization and Functional Analysis of Cellulase cbh (Cellobiohydrolase) Genes from Trichoderma spp." @default.
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