FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2483485666> ?p ?o ?g. }

• W2483485666 endingPage "gkw662" @default.
• W2483485666 startingPage "gkw662" @default.
• W2483485666 abstract "Environmental exposure, endogenous metabolism and cancer chemotherapy can give rise to alkylation of DNA, and the resulting alkylated thymidine (alkyldT) lesions were found to be poorly repaired and persistent in mammalian tissues. Unrepaired DNA lesions may compromise genomic integrity by inhibiting DNA replication and inducing mutations in these processes. In this study, we explored how eight O4-alkyldT lesions, with the alkyl group being a Me, Et, nPr, iPr, nBu, iBu, (R)-sBu and (S)-sBu, are recognized by DNA replication machinery in HEK293T human embryonic kidney cells. We found that the O4-alkyldT lesions are moderately blocking to DNA replication, with the bypass efficiencies ranging from 20 to 33% in HEK293T cells, and these lesions induced substantial frequencies T→C transition mutation. We also conducted the replication experiments in the isogenic cells where individual translesion synthesis (TLS) DNA polymerases were depleted by the CRISPR/Cas9 genome editing method. Our results showed that deficiency in Pol η or Pol ζ, but not Pol κ or Pol ι, led to pronounced drops in bypass efficiencies for all the O4-alkyldT lesions except O4-MedT. In addition, depletion of Pol ζ resulted in significant decreases in T→C mutation frequencies for all the O4-alkyldT lesions except O4-MedT and O4-nBudT. Thus, our study provided important new knowledge about the cytotoxic and mutagenic properties of the O4-alkyldT lesions and defined the roles of TLS polymerases in bypassing these lesions in human cells." @default.
• W2483485666 created "2016-08-23" @default.
• W2483485666 creator A5007733938 @default.
• W2483485666 creator A5025279909 @default.
• W2483485666 creator A5037741645 @default.
• W2483485666 creator A5076587936 @default.
• W2483485666 creator A5079804162 @default.
• W2483485666 creator A5090638122 @default.
• W2483485666 date "2016-07-27" @default.
• W2483485666 modified "2023-09-25" @default.
• W2483485666 title "Translesion synthesis of<i>O</i>⁴-alkylthymidine lesions in human cells" @default.
• W2483485666 cites W1452050606 @default.
• W2483485666 cites W1519435177 @default.
• W2483485666 cites W1651084032 @default.
• W2483485666 cites W1712429112 @default.
• W2483485666 cites W1815380513 @default.
• W2483485666 cites W1965153645 @default.
• W2483485666 cites W1969228339 @default.
• W2483485666 cites W1973268287 @default.
• W2483485666 cites W1984650264 @default.
• W2483485666 cites W1985746590 @default.
• W2483485666 cites W1987338695 @default.
• W2483485666 cites W2001209925 @default.
• W2483485666 cites W2001866815 @default.
• W2483485666 cites W2003171404 @default.
• W2483485666 cites W2009007206 @default.
• W2483485666 cites W2012012836 @default.
• W2483485666 cites W2016175366 @default.
• W2483485666 cites W2017985800 @default.
• W2483485666 cites W2021311129 @default.
• W2483485666 cites W2026178154 @default.
• W2483485666 cites W2036176224 @default.
• W2483485666 cites W2042970469 @default.
• W2483485666 cites W2046174296 @default.
• W2483485666 cites W2053012689 @default.
• W2483485666 cites W2064063117 @default.
• W2483485666 cites W2064815984 @default.
• W2483485666 cites W2066199073 @default.
• W2483485666 cites W2070172761 @default.
• W2483485666 cites W2071444261 @default.
• W2483485666 cites W2082300861 @default.
• W2483485666 cites W2085993152 @default.
• W2483485666 cites W2089084596 @default.
• W2483485666 cites W2107181486 @default.
• W2483485666 cites W2107840333 @default.
• W2483485666 cites W2122436900 @default.
• W2483485666 cites W2151552495 @default.
• W2483485666 cites W2164390042 @default.
• W2483485666 cites W2235379487 @default.
• W2483485666 cites W2322844820 @default.
• W2483485666 cites W2409417730 @default.
• W2483485666 cites W2477626371 @default.
• W2483485666 doi "https://doi.org/10.1093/nar/gkw662" @default.
• W2483485666 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5100597" @default.
• W2483485666 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27466394" @default.
• W2483485666 hasPublicationYear "2016" @default.
• W2483485666 type Work @default.
• W2483485666 sameAs 2483485666 @default.
• W2483485666 citedByCount "32" @default.
• W2483485666 countsByYear W24834856662016 @default.
• W2483485666 countsByYear W24834856662017 @default.
• W2483485666 countsByYear W24834856662019 @default.
• W2483485666 countsByYear W24834856662020 @default.
• W2483485666 countsByYear W24834856662021 @default.
• W2483485666 countsByYear W24834856662022 @default.
• W2483485666 countsByYear W24834856662023 @default.
• W2483485666 crossrefType "journal-article" @default.
• W2483485666 hasAuthorship W2483485666A5007733938 @default.
• W2483485666 hasAuthorship W2483485666A5025279909 @default.
• W2483485666 hasAuthorship W2483485666A5037741645 @default.
• W2483485666 hasAuthorship W2483485666A5076587936 @default.
• W2483485666 hasAuthorship W2483485666A5079804162 @default.
• W2483485666 hasAuthorship W2483485666A5090638122 @default.
• W2483485666 hasBestOaLocation W24834856661 @default.
• W2483485666 hasConcept C104317684 @default.
• W2483485666 hasConcept C134935766 @default.
• W2483485666 hasConcept C143425029 @default.
• W2483485666 hasConcept C153911025 @default.
• W2483485666 hasConcept C174510640 @default.
• W2483485666 hasConcept C2756471 @default.
• W2483485666 hasConcept C2777108182 @default.
• W2483485666 hasConcept C501734568 @default.
• W2483485666 hasConcept C54355233 @default.
• W2483485666 hasConcept C552990157 @default.
• W2483485666 hasConcept C64241487 @default.
• W2483485666 hasConcept C73573662 @default.
• W2483485666 hasConcept C81885089 @default.
• W2483485666 hasConcept C82381507 @default.
• W2483485666 hasConcept C86803240 @default.
• W2483485666 hasConcept C95444343 @default.
• W2483485666 hasConceptScore W2483485666C104317684 @default.
• W2483485666 hasConceptScore W2483485666C134935766 @default.
• W2483485666 hasConceptScore W2483485666C143425029 @default.
• W2483485666 hasConceptScore W2483485666C153911025 @default.
• W2483485666 hasConceptScore W2483485666C174510640 @default.
• W2483485666 hasConceptScore W2483485666C2756471 @default.
• W2483485666 hasConceptScore W2483485666C2777108182 @default.
• W2483485666 hasConceptScore W2483485666C501734568 @default.

• next