Matches in SemOpenAlex for { <https://semopenalex.org/work/W2485469884> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2485469884 abstract "To investigate the involvement of the E1A region in the restricted growth of Ad40, a preliminary characterisation of sequences which were important for basal and trans-activated transcription was undertaken. Comparison of the intact Ad40 E1A promoter with the intact Ad5 E1A promoter in transient transfection assays, revealed that basal transcription from the Ad40 E1A promoter was lowered by approximately 6 fold when compared to basal transcription from the Ad5 E1A promoter. To identify sequences important for basal transcription within the Ad40 E1A promoter, a series of promoter deletions were constructed using Bal 31 nuclease digestion, revealing a region between -349 to -140, relative to the cap site at +1, to be important for basal transcription from the Ad40 E1A promoter. Comparison of the Ad40 E1A promoter with the transcription factor database, held at EMBL (Ghosh, 1990), revealed that this region contained a number of possible transcription factor binding sites similarly arranged to the Ad5 E1A promoter. To map sequences within the region -349 to -140, a series of deletions were constructed which deleted transcription factor binding sites known to be important for basal transcription from the Ad5 E1A promoter. The deletions were characterised in parallel with the intact and Bal 31 deletion mutants of the Ad40 E1A promoter by transient transfection assays, which revealed that the Ad40 E1A transcriptional control region contains an enhancer element located between -328 to -235 relative to the Ad40 E1A cap site at +1 similar to that in the Ad5 E1A promoter (Hearing and Shenk, 1983a, 1986; Bruder and Hearing, 1989, 1991). To investigate whether the Ad40 E1A 249R (equivalent to the Ad5 E1A 13S mRNA which encodes a 289R protein) and 22IR (equivalent to the Ad5 E1A 12S mRNA which encodes a 243R protein) proteins were involved in the aberrant expression of the Ad40 E1A region, a cDNA equivalent to the Ad5 E1A 13S mRNA was generated by RT-PCR of Ad40 infected cell extracts, then cloned into a CMV expression plasmid. An Ad40 equivalent to the Ad5 E1A 12S mRNA was not generated by RT-PCR of Ad40 infected cell extracts However, a library of Ad40 E1A specific cDNAs were generated, and four novel Ad40 E1A specific cDNAs were characterised. Comparison of trans-activated transcription from the Ad40 E1A promoter by the Ad40 E1A 249R protein and the Ad5 E1A 289R protein, revealed that trans-activated transcription from the Ad40 E1A promoter was approximately 6 fold lower in the presence of the Ad40 E1A 249R protein. To map sequences which were important for trans-activated transcription from the Ad40 E1A promoter, cells were cotransfected with either the intact or deleted Ad40 E1A promoter and the Ad5 E1A 289R protein or the Ad40 E1A 249R E1A protein, revealing a region between -328 to -235, relative to the Ad40 E1A cap site at +1, to be important for Ad5 E1A 289R activated transcription from the Ad40 E1A promoter. Ad40 E1A 249R activated expression for both the Ad5 and Ad40 E5A promoters was not discussed, due to an equipment (incubator) problem which rendered the experimental data unreliable. Comparison of the ratios of Ad5 E1A 289R activated expression to basal expression within the Ad5 and Ad40 promoter constructs indicated that no single sequence element could be identified which was uniquely important for trans-activated expression. Rather those elements which affected the level of basal expression seemed to have an effect on the level of trans-activated expression observed in the presence of the Ad5 E1A 289R protein. Thus the Ad5 E1A 2S9R protein probably activates gene expression through the basal transcription apparatus, as demonstrated by the Ad5 E1A promoter. (Abstract shortened by ProQuest.)." @default.
- W2485469884 created "2016-08-23" @default.
- W2485469884 creator A5018704608 @default.
- W2485469884 date "2000-01-01" @default.
- W2485469884 modified "2023-09-27" @default.
- W2485469884 title "Preliminary characterisation of the adenovirus type 40 E1A region" @default.
- W2485469884 hasPublicationYear "2000" @default.
- W2485469884 type Work @default.
- W2485469884 sameAs 2485469884 @default.
- W2485469884 citedByCount "0" @default.
- W2485469884 crossrefType "dissertation" @default.
- W2485469884 hasAuthorship W2485469884A5018704608 @default.
- W2485469884 hasConcept C101762097 @default.
- W2485469884 hasConcept C104317684 @default.
- W2485469884 hasConcept C111936080 @default.
- W2485469884 hasConcept C138885662 @default.
- W2485469884 hasConcept C143065580 @default.
- W2485469884 hasConcept C150194340 @default.
- W2485469884 hasConcept C153911025 @default.
- W2485469884 hasConcept C179926584 @default.
- W2485469884 hasConcept C2776954459 @default.
- W2485469884 hasConcept C40767141 @default.
- W2485469884 hasConcept C41895202 @default.
- W2485469884 hasConcept C54355233 @default.
- W2485469884 hasConcept C86339819 @default.
- W2485469884 hasConcept C86803240 @default.
- W2485469884 hasConceptScore W2485469884C101762097 @default.
- W2485469884 hasConceptScore W2485469884C104317684 @default.
- W2485469884 hasConceptScore W2485469884C111936080 @default.
- W2485469884 hasConceptScore W2485469884C138885662 @default.
- W2485469884 hasConceptScore W2485469884C143065580 @default.
- W2485469884 hasConceptScore W2485469884C150194340 @default.
- W2485469884 hasConceptScore W2485469884C153911025 @default.
- W2485469884 hasConceptScore W2485469884C179926584 @default.
- W2485469884 hasConceptScore W2485469884C2776954459 @default.
- W2485469884 hasConceptScore W2485469884C40767141 @default.
- W2485469884 hasConceptScore W2485469884C41895202 @default.
- W2485469884 hasConceptScore W2485469884C54355233 @default.
- W2485469884 hasConceptScore W2485469884C86339819 @default.
- W2485469884 hasConceptScore W2485469884C86803240 @default.
- W2485469884 hasLocation W24854698841 @default.
- W2485469884 hasOpenAccess W2485469884 @default.
- W2485469884 hasPrimaryLocation W24854698841 @default.
- W2485469884 hasRelatedWork W1444481305 @default.
- W2485469884 hasRelatedWork W1551272921 @default.
- W2485469884 hasRelatedWork W1577005712 @default.
- W2485469884 hasRelatedWork W1598941499 @default.
- W2485469884 hasRelatedWork W1800609782 @default.
- W2485469884 hasRelatedWork W1862281779 @default.
- W2485469884 hasRelatedWork W1898859148 @default.
- W2485469884 hasRelatedWork W1964229112 @default.
- W2485469884 hasRelatedWork W1982894038 @default.
- W2485469884 hasRelatedWork W1998912417 @default.
- W2485469884 hasRelatedWork W2011855448 @default.
- W2485469884 hasRelatedWork W2027878246 @default.
- W2485469884 hasRelatedWork W2035512546 @default.
- W2485469884 hasRelatedWork W2037260804 @default.
- W2485469884 hasRelatedWork W2039836699 @default.
- W2485469884 hasRelatedWork W2071039810 @default.
- W2485469884 hasRelatedWork W2079569052 @default.
- W2485469884 hasRelatedWork W2091776071 @default.
- W2485469884 hasRelatedWork W2158392948 @default.
- W2485469884 hasRelatedWork W3028751811 @default.
- W2485469884 isParatext "false" @default.
- W2485469884 isRetracted "false" @default.
- W2485469884 magId "2485469884" @default.
- W2485469884 workType "dissertation" @default.